Rabbit anti-Human TM165 Polyclonal Antibody | anti-Tmem165 antibody

TM165, NT (TMEM165, TPARL, Transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL)

Cat. Num. AAA6002950

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TM165; Polyclonal Antibody; NT (TMEM165; TPARL; Transmembrane protein 165; Transmembrane protein PT27; Transmembrane protein TPARL); Anti -TM165; anti-Tmem165 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Tmem165 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: TM165 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human TM165.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TM165 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TM165 antibody detected the TM165 protein (arrow).)

Flow Cytometry (FC/FACS)

(TM165 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Tmem165 antibody

The function of TM165 remains unknown.

Product Categories/Family for anti-Tmem165 antibody

Antibodies; Abs to Transmembrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 475598931
• Molecular Weight: 34,791 Da
• NCBI Official Full Name: Transmembrane protein 165
• UniProt Protein Name: Transmembrane protein 165
• Protein Family: Transmembrane protein
• UniProt Gene Name: Tmem165
• UniProt Synonym Gene Names: Tparl
• UniProt Entry Name: TM165_MOUSE

Uniprot Description

TMEM165: Defects in TMEM165 are the cause of congenital disorder of glycosylation type 2K (CDG2K). An autosomal recessive disorder with a variable phenotype. Affected individuals show psychomotor retardation and growth retardation, and most have short stature. Other features include dysmorphism, hypotonia, eye abnormalities, acquired microcephaly, hepatomegaly, and skeletal dysplasia. Congenital disorders of glycosylation are caused by a defect in glycoprotein biosynthesis and characterized by under- glycosylated serum glycoproteins and a wide variety of clinical features. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the GDT1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; intracellular membrane-bound organelle; lysosome; lysosomal membrane; integral to membrane; endosome membrane; trans-Golgi network membrane; endosome

Biological Process: cellular calcium ion homeostasis; protein amino acid N-linked glycosylation; Golgi calcium ion transport