Rabbit anti-Human TM165 Polyclonal Antibody | anti-TM165 antibody

TM165, NT (TMEM165, TPARL, Transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL) (FITC)

Cat. Num. AAA6355979

• Gene Names: TMEM165; FT27; GDT1; CDG2K; TPARL; TMPT27
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TM165; Polyclonal Antibody; NT (TMEM165; TPARL; Transmembrane protein 165; Transmembrane protein PT27; Transmembrane protein TPARL) (FITC); TMEM165; Transmembrane protein TPARL; anti-TM165 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TM165 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TM165 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human TM165.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TM165 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TM165 antibody detected the TM165 protein (arrow).)

Flow Cytometry (FC/FACS)

(TM165 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TM165 antibody

Antibodies; Transmembrane Proteins

References

Terracciano, A., et al. Mol. Psychiatry (2008). Hu, R.M., et al. Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548(2000). Akagi, J., et al. Biochem. Biophys. Res. Commun. 157(2):548-557(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 32189371
• NCBI GeneID: 55858
• NCBI Accession #: NP_060945.2
• NCBI GenBank Nucleotide #: NM_018475.4
• UniProt Accession #: Q9HC07; Q9BTN9; Q9NZ34; A8K3P8; B4DHW1
• Molecular Weight: 28,432 Da
• NCBI Official Full Name: transmembrane protein 165
• NCBI Official Synonym Full Names: transmembrane protein 165
• NCBI Official Symbol: TMEM165
• NCBI Official Synonym Symbols: FT27; GDT1; CDG2K; TPARL; TMPT27
• NCBI Protein Information: transmembrane protein 165; TPA regulated locus; transmembrane protein PT27; transmembrane protein TPARL
• UniProt Protein Name: Transmembrane protein 165
• UniProt Gene Name: TMEM165
• UniProt Synonym Gene Names: TPARL
• UniProt Entry Name: TM165_HUMAN

NCBI Description

This gene encodes a predicted transmembrane protein with a perinuclear Golgi-like distribution in fibroblasts. Mutations in this gene are associated with the autosomal recessive disorder congenital disorder of glycosylation, type IIk. Knockdown of this gene's expression causes decreased sialylation in HEK cells and suggests this gene plays a role in terminal Golgi glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]