TLR7 Polyclonal Antibody | anti-TLR7 antibody

Anti-TLR7 Antibody

Cat. Num. AAA178048

• Gene Names: TLR7; TLR7-like
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TLR7; Polyclonal Antibody; Anti-TLR7 Antibody; Toll-like receptor 7; PRO285; TLR 7; Tlr7; TLR7_HUMAN; Toll like receptor 7; UNQ248 antibody; toll-like receptor 7; anti-TLR7 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1049

• Applicable Applications for anti-TLR7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunohistochemistry (IHC) Formalin Concentration: 0.5-1ug/ml; Immunocytochemistry Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- TLR7 Picoband antibody, MBS178048, Western blottingAll lanes: Anti TLR7 (MBS178048) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 121KDObserved bind size: 121KD )

Immunohistochemistry (IHC)

(Anti- TLR7 Picoband antibody, MBS178048, IHC(P)IHC(P): Mouse Thymus Tissue )

Immunohistochemistry (IHC)

(Anti- TLR7 Picoband antibody, MBS178048, IHC(P)IHC(P): Rat Thymus Tissue )

Immunohistochemistry (IHC)

(Anti- TLR7 Picoband antibody, MBS178048, IHC(P)IHC(P): Human Placenta Tissue )

Immunohistochemistry (IHC)

(Anti- TLR7 Picoband antibody, MBS178048, IHC(F)IHC(F): Human Placenta Tissue )

Immunocytochemistry (ICC)

(Anti- TLR7 Picoband antibody, MBS178048, ICCICC: A549 Cell )

Related Product Information for anti-TLR7 antibody

Description: Rabbit IgG polyclonal antibody for Toll-like receptor 7(TLR7) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Background: TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.

References

1. Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., Reis e Sousa, C.Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA.Science 303: 1529-1531, 2004. 2. Hayashi, T., Gray, C. S., Chan, M., Tawatao, R., Ronacher, L., McGargill, M. A., Datta, S. K., Carson, D. A., Corr, M.Prevention of autoimmune disease by induction of tolerance to Toll-like receptor 7. 3. Hemmi, H., Kaisho, T., Takeuchi, O., Sato, S., Sanjo, H., Hoshino, K., Horiuchi, T., Tomizawa, H., Takeda, K., Akira, S.Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway.Nature Immun. 3: 196-200, 2002.

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• Molecular Weight: 120,922 Da
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; endosome membrane; Golgi membrane; integral to plasma membrane; lysosome; plasma membrane; receptor complex

Molecular Function: double-stranded RNA binding; drug binding; receptor activity; single-stranded RNA binding; siRNA binding; transmembrane receptor activity

Biological Process: defense response to virus; I-kappaB phosphorylation; inflammatory response; innate immune response; microglial cell activation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of chemokine production; positive regulation of inflammatory response; positive regulation of interferon-alpha biosynthetic process; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of NF-kappaB import into nucleus; regulation of protein amino acid phosphorylation; toll-like receptor 7 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway