Standard Curve (Sample)
Goat Copper chaperone for superoxide dismutase (CCS) ELISA Kit | CCS elisa kit
Goat Copper chaperone for superoxide dismutase (CCS) ELISA Kit
Reactivity
Goat
Synonyms
Copper chaperone for superoxide dismutase (CCS); N/A; Goat Copper chaperone for superoxide dismutase (CCS) ELISA Kit; CCS elisa kit
Reactivity
Goat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ug/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCS elisa kit
Intended Uses: This CCS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CCS. This ELISA kit for research use only!
Principle of the Assay: CCS ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CCS antibody and an CCS-HRP conjugate. The assay sample and buffer are incubated together with CCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCS concentration since CCS from samples and CCS-HRP conjugate compete for the anti-CCS antibody binding site. Since the number of sites is limited, as more sites are occupied by CCS from the sample, fewer sites are left to bind CCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCS concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CCS ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CCS antibody and an CCS-HRP conjugate. The assay sample and buffer are incubated together with CCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCS concentration since CCS from samples and CCS-HRP conjugate compete for the anti-CCS antibody binding site. Since the number of sites is limited, as more sites are occupied by CCS from the sample, fewer sites are left to bind CCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCS concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,041 Da
NCBI Official Full Name
copper chaperone for superoxide dismutase
NCBI Official Synonym Full Names
copper chaperone for superoxide dismutase
NCBI Official Symbol
CCS
NCBI Protein Information
copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone
UniProt Protein Name
Copper chaperone for superoxide dismutase
UniProt Gene Name
CCS
UniProt Entry Name
CCS_HUMAN
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Product Notes
The Goat CCS ccs (Catalog #AAA245228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245228 ELISA Kit recognizes Goat CCS. It is sometimes possible for the material contained within the vial of "Copper chaperone for superoxide dismutase (CCS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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