Mouse Kruppel Like Factor 1, Erythroid (KLF1) ELISA Kit | KLF1 elisa kit

Mouse Kruppel Like Factor 1, Erythroid (KLF1) ELISA Kit

Cat. Num. AAA2021293

• Gene Names: KLF1; EKLF; INLU; CDAN4; HBFQTL6
• Reactivity: Mouse
• Synonyms: Kruppel Like Factor 1; Erythroid (KLF1); Mouse Kruppel Like Factor 1; Erythroid (KLF1) ELISA Kit; EKLF; Erythroid krueppel-like transcription factor; KLF1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kruppel Like Factor 1, Erythroid (KLF1).; No significant cross-reactivity or interference between Kruppel Like Factor 1, Erythroid (KLF1) and analogues was observed.
• Sequence Length: 362

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kruppel Like Factor 1, Erythroid (KLF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kruppel Like Factor 1, Erythroid (KLF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 1, Erythroid (KLF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 1, Erythroid (KLF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 1, Erythroid (KLF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kruppel Like Factor 1, Erythroid (KLF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KLF1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 10835246
• NCBI GeneID: 10661
• NCBI Accession #: NP_006554.1
• NCBI GenBank Nucleotide #: NM_006563.3
• UniProt Accession #: Q13351; Q6PIJ5; Q92899
• Molecular Weight: 38,221 Da
• NCBI Official Full Name: Krueppel-like factor 1
• NCBI Official Synonym Full Names: Kruppel-like factor 1 (erythroid)
• NCBI Official Symbol: KLF1
• NCBI Official Synonym Symbols: EKLF; INLU; CDAN4; HBFQTL6
• NCBI Protein Information: Krueppel-like factor 1
• UniProt Protein Name: Krueppel-like factor 1
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF1
• UniProt Synonym Gene Names: EKLF; EKLF
• UniProt Entry Name: KLF1_HUMAN

NCBI Description

This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]

Uniprot Description

KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nuclear chromatin; nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: chromatin remodeling; erythrocyte maturation; transcription, DNA-dependent; embryonic hemopoiesis; in utero embryonic development; positive regulation of transcription, DNA-dependent; erythrocyte differentiation; liver development

Disease: Fetal Hemoglobin Quantitative Trait Locus 6; Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor