Rabbit anti-Human, Mouse TLR7 Polyclonal Antibody | anti-Tlr7 antibody

TLR7 Antibody

Cat. Num. AAA150192

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: TLR7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TLR7; Polyclonal Antibody; TLR7 Antibody; Toll-like receptor 7; toll-like receptor 7; anti-Tlr7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TLR7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1050

• Applicable Applications for anti-Tlr7 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TLR7 antibody was raised against a peptide corresponding to 14 amino acids near the center of mouse TLR7.
• Buffer: TLR7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TLR7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 μg/mL.)

Related Product Information for anti-Tlr7 antibody

TLR7 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFNalpha secretion, suggesting the importance of TLR7 in virus recognition.

NCBI and Uniprot Product Information

• NCBI GI #: 18875360
• NCBI GeneID: 170743
• NCBI Accession #: NP_573474
• NCBI GenBank Nucleotide #: NM_133211.4
• UniProt Accession #: P58681; Q923I1
• Molecular Weight: Predicted: 116 kDa; ; Observed: 135 kDa
• NCBI Official Full Name: toll-like receptor 7 isoform a
• NCBI Official Synonym Full Names: toll-like receptor 7
• NCBI Official Symbol: Tlr7
• NCBI Protein Information: toll-like receptor 7
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr7
• UniProt Entry Name: TLR7_MOUSE

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; lysosome; cytoplasm; plasma membrane; integral to membrane; cytoplasmic vesicle; endosome; receptor complex

Molecular Function: protein binding; single-stranded RNA binding; siRNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding

Biological Process: regulation of protein amino acid phosphorylation; immune system process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 7 signaling pathway; signal transduction; positive regulation of chemokine production; positive regulation of interleukin-8 production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; immune response; inflammatory response; I-kappaB phosphorylation; defense response to virus; positive regulation of interferon-gamma biosynthetic process