Rabbit anti-Human TLR6 Polyclonal Antibody | anti-TLR6 antibody

TLR6 Antibody

Cat. Num. AAA150949

• Gene Names: TLR6; CD286
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: TLR6 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TLR6; Polyclonal Antibody; TLR6 Antibody; CD286; Toll-like receptor 6; toll-like receptor 6; anti-TLR6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TLR6 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 796

• Applicable Applications for anti-TLR6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: TLR6 antibody can be used for detection of TLR6 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TLR6 antibody was raised against a peptide corresponding to 13 amino acids near the center of human TLR6.
• Buffer: TLR6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TLR6 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TLR6 in Jurkat cell lysate with TLR6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of TLR6 in Jurkat cells with TLR6 antibody at 2 μg/mL.)

Related Product Information for anti-TLR6 antibody

TLR6 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR6 was first identified as a close homolog of TLR1, sharing 69% sequence identify. Like TLR1, TLR6 can form heterodimers with TLR2, and these TLR6:TLR2 dimers coordinate macrophage activation by Gram-positive bacteria and the yeast cell wall particle zymosan. Activation of these complexes not only initiates pro-inflammatory cascades, but also can lead to apoptotic responses.

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059
• NCBI GenBank Nucleotide #: NM_006068.4
• UniProt Accession #: Q9Y2C9; Q2NKL3; B3Y640; B6CH35; B6RFS4; B6RFS5
• Molecular Weight: 55,828 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll-like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: TLR6
• UniProt Entry Name: TLR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

TLR6: Participates in the innate immune response to Gram- positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of JNK activity; T-helper 1 type immune response; regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; detection of diacylated bacterial lipoprotein; positive regulation of interleukin-6 biosynthetic process; activation of NF-kappaB-inducing kinase; signal transduction; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to bacterium; toll-like receptor 6 signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; inflammatory response; toll-like receptor 4 signaling pathway