Rabbit TLR4 Polyclonal Antibody | anti-TLR4 antibody

TLR4 Antibody (Center)

Cat. Num. AAA9206134

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Reactivity: Human; Predicted Reactivity: Bovine, Hamster, Horse, Pig
• Applications: Western Blot, ELISA, Flow Cytometry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TLR4; Polyclonal Antibody; TLR4 Antibody (Center); Toll-like receptor 4; hToll; CD284; anti-TLR4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Hamster, Horse, Pig
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-698 amino acids from the Central region of human TLR4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 669-698
• Sequence Length: 639

• Applicable Applications for anti-TLR4 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Funtion: Cooperates with LY96 and CD14 to mediatethe innate immune response to bacteriallipopolysaccharide (LPS) (PubMed:27022195). Actsvia MYD88, TIRAP and TRAF6, leading toNF-kappa-B activation, cytokine secretionand the inflammatory response (PubMed:9237759,PubMed:10835634,PubMed:27022195). Alsoinvolved in LPS-independent inflammatoryresponses triggered by free fatty acids,such as palmitate, and Ni(2+). Responsestriggered by Ni(2+) require non- conservedhistidines and are, therefore,species-specific (PubMed:20711192). BothM.tuberculosis HSP70 (dnaK) and HSP65(groEL-2) act via this protein to stimulateNF-kappa-B expression (PubMed:15809303). Incomplex with TLR6, promotes sterileinflammation in monocytes/macrophages inresponse to oxidized low-density lipoprotein(oxLDL) or amyloid-beta 42. In this context,the initial signal is provided by oxLDL- oramyloid-beta 42-binding to CD36. Thisevent induces the formation of aheterodimer of TLR4 and TLR6, which israpidly internalized and triggersinflammatory response, leading to theNF-kappa-B-dependent production ofCXCL1, CXCL2 and CCL9 cytokines, viaMYD88 signaling pathway, and CCL5cytokine, via TICAM1 signaling pathway, aswell as IL1B secretion. Bindselectronegative LDL (LDL(-)) and mediatesthe cytokine release induced by LDL(-)(PubMed:23880187).Stimulation of monocytes in vitro withM.tuberculosis PstS1 induces p38 MAPK andERK1/2 activation primarily via TLR2, butalso partially via this receptor (PubMed:16622205).
• Cellular Location: Cell membrane; Single-pass type Imembrane protein. Early endosome. Cellprojection, ruffle{ECO:0000250|UniProtKB:Q9QUK6}.Note=Upon complex formation with CD36and TLR6, internalized throughdynamin-dependent endocytosis(PubMed:20037584). Colocalizes withRFTN1 at cell membrane and then togetherwith RFTN1 moves to endosomes, uponlipopolysaccharide stimulation.
• Tissue Location: Highly expressed in placenta, spleen andperipheral blood leukocytes(PubMed:9435236, PubMed:9237759).Detected in monocytes, macrophages,dendritic cells and several types of T-cells(PubMed:9237759, PubMed:27022195).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of TLR4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). TLR4 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(TLR4 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TLR4 antibody

TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.

Product Categories/Family for anti-TLR4 antibody

Cardiovascular; Immunology; Microbiology; Signal Transduction

References

Sam-Agudu, N.A., et al. Am. J. Trop. Med. Hyg. 82(4):548-555(2010)
# Palomino-Morales, R.J., et al. Arthritis Res. Ther. 12 (2), R51 (2010)
# Rigoli, L., et al. Anticancer Res. 30(2):513-517(2010)
# da Silva Correia, J., et al. J. Biol. Chem. 277(3):1845-1854(2002)
# Shimazu, R., et al. J. Exp. Med. 189(11):1777-1782(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 4507533
• NCBI GeneID: 7099
• NCBI Accession #: NP_003257.1; NP_612564.1; NP_612567.1
• NCBI GenBank Nucleotide #: NM_003266.3
• UniProt Accession #: O00206; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53
• NCBI Official Full Name: toll-like receptor 4 isoform C
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR4
• UniProt Entry Name: TLR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: integral to plasma membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; endosome membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; negative regulation of interleukin-6 production; positive regulation of B cell proliferation; positive regulation of interferon-beta production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; T-helper 1 type immune response; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; macrophage activation; negative regulation of interleukin-23 production; detection of lipopolysaccharide; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of osteoclast differentiation; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of JNK cascade; defense response to Gram-negative bacterium; positive regulation of interleukin-8 production; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; lipopolysaccharide-mediated signaling pathway; interferon-gamma production; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; detection of fungus; MyD88-independent toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of interleukin-1 production; positive regulation of inflammatory response

Disease: Macular Degeneration, Age-related, 10