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ELISA (EIA) (Sandwich ELISA analysis of human IFN beta using Mouse anti Human IFN beta as a capture reagent and biotinylated Mouse anti Human IFN beta (MBS226155) as a detection reagent with purified recombinant IFN beta as the antigen. Detection was perfor)

Mouse anti-Human IFN Beta Monoclonal Antibody | anti-IFNBETA antibody

Mouse anti Human IFN Beta:Biotin

Gene Names
IFNB1; IFB; IFF; IFNB
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
IFN Beta; Monoclonal Antibody; Mouse anti Human IFN Beta:Biotin; IFN Beta antibody | C06-4F6; anti-IFNBETA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
C06-4F6
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-IFNBETA antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli derived recombinant human full length IFN beta (Met22-Asn187)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human IFN beta using Mouse anti Human IFN beta as a capture reagent and biotinylated Mouse anti Human IFN beta (MBS226155) as a detection reagent with purified recombinant IFN beta as the antigen. Detection was perfor)

ELISA (EIA) (Sandwich ELISA analysis of human IFN beta using Mouse anti Human IFN beta as a capture reagent and biotinylated Mouse anti Human IFN beta (MBS226155) as a detection reagent with purified recombinant IFN beta as the antigen. Detection was perfor)
Related Product Information for anti-IFNBETA antibody
Mouse anti Human IFN beta antibody, clone C06-4F6, recognizes interferon beta (IFN beta) also known as fibroblast interferon, a type I interferon. It is constitutively expressed, but is synthesized at a higher level upon infection (Takaoka & Taniguchi 2003). IFN beta plays many roles within inflammation including limiting the immune response during inflammation through induction of IL-10 and can inhibit the inflammasome and IL-1 beta. It can also play a role in the reprogramming of macrophages to an inflammatory and pro-resolving phenotype (Satyanarayanan et al. 2019). These responses are mediated through its interaction with the IFN alpha/beta receptor with subsequent downstream activation of STAT1, 2 or 3 (Takaoka & Taniguchi 2003).

Beyond inflammation IFN beta has been observed to block cancer progression by reducing the recruitment of pro-angiogenic neutrophils into tumors (Andzinski et al. 2015).

The biotinylated Mouse anti Human IFN beta antibody, clone C06-4F6 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human IFN beta antibody, clone F03-2G4 as the capture antibody.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta; IFN-beta; fibroblast interferon
UniProt Protein Name
Interferon beta
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response

Research Articles on IFNBETA

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Product Notes

The IFNBETA ifnb1 (Catalog #AAA226155) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human IFN Beta:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFN Beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the IFNBETA ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN Beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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