Rabbit anti-Human TLR4 Polyclonal Antibody | anti-TLR4 antibody

TLR4, ID (TLR4, Toll-like receptor 4, hToll, CD284) (APC)

Cat. Num. AAA6355889

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TLR4; Polyclonal Antibody; ID (TLR4; Toll-like receptor 4; hToll; CD284) (APC); CD284; anti-TLR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TLR4.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TLR4 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 669-698 in the central region of human TLR4, UniProt Accession: #O00206. Species sequence homology: bovine, hamster, porcine, equine
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis (Center) in HL-60 cell line lysates (35ug/lane) using 042879. TLR4 (arrow) was detected using 042879.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram) using 042879. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TLR4 antibody

TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.

Product Categories/Family for anti-TLR4 antibody

Antibodies; Toll Like Receptors (TLR)

References

Sam-Agudu, N.A., et al. Am. J. Trop. Med. Hyg. 82(4):548-555(2010). Palomino-Morales, R.J., et al. Arthritis Res. Ther. 12 (2), R51 (2010). Rigoli, L., et al. Anticancer Res. 30(2):513-517(2010). da Silva Correia, J., et al. J. Biol. Chem. 277(3):1845-1854(2002). Shimazu, R., et al. J. Exp. Med. 189(11):1777-1782(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4507533
• NCBI GeneID: 7099
• NCBI Accession #: NP_003257.1
• NCBI GenBank Nucleotide #: NM_003266.3
• UniProt Accession #: O00206; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194
• Molecular Weight: 95,680 Da
• NCBI Official Full Name: toll-like receptor 4 isoform C
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4; hToll; homolog of Drosophila toll
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR4
• UniProt Entry Name: TLR4_HUMAN