Mouse Catenin Beta 1 (CTNNb1) ELISA Kit | CTNNb1 elisa kit

Mouse Catenin Beta 1 (CTNNb1) ELISA Kit

Cat. Num. AAA2020486

• Gene Names: CTNNB1; CTNNB; MRD19; armadillo
• Reactivity: Mouse
• Synonyms: Catenin Beta 1 (CTNNb1); Mouse Catenin Beta 1 (CTNNb1) ELISA Kit; bCat; b-Cat; CTNN-B1; Cadherin-Associated Protein; CTNNb1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1).; No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
• Sequence Length: 781

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTNNb1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Beta 1 (CTNNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Beta 1 (CTNNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Beta 1 (CTNNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Beta 1 (CTNNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTNNb1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4503131
• NCBI GeneID: 1499
• NCBI Accession #: NP_001895.1
• NCBI GenBank Nucleotide #: NM_001904.3
• UniProt Accession #: P35222; Q8NEW9; Q8NI94; Q9H391; A8K1L7
• Molecular Weight: 9,501 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), beta 1, 88kDa
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: CTNNB; MRD19; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB
• UniProt Entry Name: CTNB1_HUMAN

NCBI Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Cell adhesion; Nuclear receptor co-regulator; Transcription factor; Oncoprotein; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: centrosome; basolateral plasma membrane; intercellular junction; fascia adherens; beta-catenin destruction complex; cytosol; transcription factor complex; cell-cell adherens junction; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; synapse; dendritic shaft; lateral plasma membrane; spindle pole; focal adhesion; tight junction; catenin complex; cell cortex; Z disc; nucleoplasm; adherens junction; apical part of cell; microvillus membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein C-terminus binding; transcription coactivator activity; transcription factor binding; protein phosphatase binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; enzyme binding; androgen receptor binding; cadherin binding; double-stranded DNA binding; protein complex binding; estrogen receptor binding; SMAD binding; nitric-oxide synthase binding; transcription factor activity; kinase binding; alpha-catenin binding; nuclear hormone receptor binding

Biological Process: regulation of myelination; regulation of centriole-centriole cohesion; positive regulation of apoptosis; protein heterooligomerization; regulation of fibroblast proliferation; positive regulation of transcription, DNA-dependent; cell maturation; negative regulation of chondrocyte differentiation; T cell differentiation in the thymus; positive regulation of fibroblast growth factor receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; osteoclast differentiation; cell-cell adhesion; positive regulation of endothelial cell differentiation; embryonic foregut morphogenesis; regulation of cell fate specification; positive regulation of mesenchymal cell proliferation; synapse organization and biogenesis; ectoderm development; male genitalia development; cell adhesion; bone resorption; response to drug; positive regulation of neuroblast proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; regulation of smooth muscle cell proliferation; hair cell differentiation; negative regulation of protein sumoylation; genitalia morphogenesis; patterning of blood vessels; muscle cell differentiation; midgut development; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; oocyte development; embryonic forelimb morphogenesis; negative regulation of osteoclast differentiation; glial cell fate determination; endodermal cell fate commitment; apoptosis; cell-matrix adhesion; neuron migration; dorsal/ventral axis specification; cell fate specification; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; response to estradiol stimulus; negative regulation of cell proliferation; central nervous system vasculogenesis; positive regulation of MAPKKK cascade; pancreas development; positive regulation of interferon type I production; fallopian tube development; layer formation in the cerebral cortex; proximal/distal pattern formation; negative regulation of mitotic cell cycle, embryonic; cell structure disassembly during apoptosis; hair follicle morphogenesis; Wnt receptor signaling pathway; thymus development; in utero embryonic development; embryonic axis specification; regulation of T cell proliferation; stem cell maintenance; neural plate development; synaptic vesicle transport; gastrulation with mouth forming second; liver development; regulation of angiogenesis; odontogenesis of dentine-containing teeth; myoblast differentiation; negative regulation of oligodendrocyte differentiation; Schwann cell proliferation; positive regulation of osteoblast differentiation; response to cadmium ion; ureteric bud branching; response to cytokine stimulus; androgen receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; epithelial to mesenchymal transition; embryonic heart tube development; lens morphogenesis in camera-type eye; anterior/posterior axis specification

Disease: Pilomatrixoma; Mental Retardation, Autosomal Dominant 19; Ovarian Cancer; Colorectal Cancer; Hepatocellular Carcinoma