Rabbit anti-Human TLR1 Polyclonal Antibody | anti-TLR1 antibody

TLR1 Antibody

Cat. Num. AAA9402959

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TLR1; Polyclonal Antibody; TLR1 Antibody; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012; anti-TLR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TLR1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 786

• Applicable Applications for anti-TLR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TLR1.
• Target Name: TLR1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TLR1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using TLR1 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using TLR1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-TLR1 antibody

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IkB that normally maintains NF-kB inactivity by sequestering it in the cytoplasm.

Product Categories/Family for anti-TLR1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 41350337
• NCBI GeneID: 7096
• NCBI Accession #: NP_003254.2
• NCBI GenBank Nucleotide #: NM_003263.3
• UniProt Accession #: Q15399; O15452; Q32MK3; Q32MK4; Q9UG90; D1CS39; D1CS41
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: toll-like receptor 1
• NCBI Official Synonym Full Names: toll-like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL
• UniProt Entry Name: TLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity

Biological Process: MyD88-dependent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; macrophage activation; regulation of cytokine secretion; positive regulation of interleukin-6 biosynthetic process; innate immune response; immune response; detection of triacylated bacterial lipoprotein; signal transduction; inflammatory response; toll-like receptor 1 signaling pathway

Disease: Leprosy, Susceptibility To, 5