Immunofluorescence (IF)
(MBS9602257 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human TLE4 Polyclonal Antibody | anti-TLE4 antibody
TLE4 Antibody
Gene Names
TLE4; ESG; BCE1; ESG4; GRG4; BCE-1; Grg-4; E(spI)
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TLE4; Polyclonal Antibody; TLE4 Antibody; B lymphocyte gene 1; E(sp1) homolog Drosophila; E(spI); enhancer of split groucho 4; ESG; ESG4; GRG4; KIAA1261; TIM; TIM1; timeless (Drosophila) homolog; Transducin like enhancer protein 4; transducin-like enhancer of split 4; anti-TLE4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TLE4 antibody detects endogenous levels of total TLE4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
805
Applicable Applications for anti-TLE4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human TLE4
Subcellular Location
Nuclear
Tissue Specificity
In all tissues examined, mostly in brain, and muscle.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Bovine (90%), Horse (90%), Sheep (90%), Rabbit (90%), Dog (100%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602257 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602257 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of TLE4 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of TLE4 using Jurkat whole cell lysates)
Related Product Information for anti-TLE4 antibody
Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).
Subunit Structure: Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Interacts with SIX3 and SIX6 (By similarity). Interacts with PAX2.
Post-translational Modifications: Phosphorylated. PAX5 binding increases phosphorylation (By similarity). Ubiquitinated by XIAP/BIRC4.
Similarity: WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain. Belongs to the WD repeat Groucho/TLE family.
Subunit Structure: Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Interacts with SIX3 and SIX6 (By similarity). Interacts with PAX2.
Post-translational Modifications: Phosphorylated. PAX5 binding increases phosphorylation (By similarity). Ubiquitinated by XIAP/BIRC4.
Similarity: WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain. Belongs to the WD repeat Groucho/TLE family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 83 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
transducin-like enhancer protein 4 isoform 1
NCBI Official Synonym Full Names
transducin like enhancer of split 4
NCBI Official Symbol
TLE4
NCBI Official Synonym Symbols
ESG; BCE1; ESG4; GRG4; BCE-1; Grg-4; E(spI)
NCBI Protein Information
transducin-like enhancer protein 4
UniProt Protein Name
Transducin-like enhancer protein 4
Protein Family
UniProt Gene Name
TLE4
UniProt Synonym Gene Names
GRG4; KIAA1261
Uniprot Description
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression ().
Research Articles on TLE4
1. Genes TGFB1, TLE4 and MUC22 are associated with the risk of childhood asthma in Chinese population.
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Product Notes
The TLE4 tle4 (Catalog #AAA9602257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLE4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLE4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TLE4 tle4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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