Rabbit anti-Human TLE4 Polyclonal Antibody | anti-TLE4 antibody

TLE4, NT (TLE4, KIAA1261, Transducin-like enhancer protein 4)

Cat. Num. AAA646673

• Gene Names: TLE4; ESG; BCE1; ESG4; GRG4; BCE-1; E(spI)
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TLE4; Polyclonal Antibody; NT (TLE4; KIAA1261; Transducin-like enhancer protein 4); Anti -TLE4; anti-TLE4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TLE4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: TLE4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-196 amino acids from the N-terminal region of human TLE4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TLE4 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the TLE4 antibody detected the TLE4 protein (arrow).)

Related Product Information for anti-TLE4 antibody

TLE4 is a transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

Product Categories/Family for anti-TLE4 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 544224628
• NCBI GeneID: 7091
• NCBI Accession #: NP_001269677.1
• NCBI GenBank Nucleotide #: NM_001282748.1
• UniProt Accession #: Q04727; Q3ZCS1; Q5T1Y2; Q6PCB3; Q9BZ07; Q9BZ08; Q9BZ09; Q9NSL3; Q9ULF9
• Molecular Weight: 83,755 Da
• NCBI Official Full Name: transducin-like enhancer protein 4 isoform 1
• NCBI Official Synonym Full Names: transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
• NCBI Official Symbol: TLE4
• NCBI Official Synonym Symbols: ESG; BCE1; ESG4; GRG4; BCE-1; E(spI)
• NCBI Protein Information: transducin-like enhancer protein 4; B lymphocyte gene 1; enhancer of split groucho 4; transducin-like enhancer of split 4, homolog of Drosophila E(sp1)
• UniProt Protein Name: Transducin-like enhancer protein 4
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE4
• UniProt Synonym Gene Names: KIAA1261
• UniProt Entry Name: TLE4_HUMAN

Uniprot Description

Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

By similarity.

Subunit structure: Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression

By similarity.

Subcellular location: Nucleus.

Tissue specificity: In all tissues examined, mostly in brain, and muscle.

Post-translational modification: Phosphorylated. PAX5 binding increases phosphorylation

By similarity.Ubiquitinated by XIAP/BIRC4. Ref.12

Sequence similarities: Belongs to the WD repeat Groucho/TLE family.Contains 7 WD repeats.

Sequence caution: The sequence BAA86575.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC22598.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAC22599.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI13644.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI13734.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI41315.1 differs from that shown. Reason: Erroneous gene model prediction.