Western Blot (WB)
(Western Blot:Sample: Recombinant TF, Rabbit.)
Guinea pig Tissue Factor (TF) Polyclonal Antibody | anti-TF antibody
Polyclonal Antibody to Tissue Factor (TF)
Reactivity
Oryctolagus cuniculus (Rabbit)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Tissue Factor (TF); Polyclonal Antibody; Polyclonal Antibody to Tissue Factor (TF); anti-TF antibody
Host
Guinea pig
Reactivity
Oryctolagus cuniculus (Rabbit)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.52mg/ml (varies by lot)
Applicable Applications for anti-TF antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Tissue Factor (Ala33~Ala292 (Accession # P24055)) expressed in E.coli (MBS2010916).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Recombinant TF, Rabbit.)
Western Blot (WB)
(Western Blot:Sample: Recombinant TF, Rabbit.)
Western Blot (WB)
(Western Blot;Sample:Lane 1: Rabbit Thymus lysate;Lane 2: Rabbit Testis lysate;Lane 3: Rabbit Lung lysatePrimary Ab: 2ug/ml Cavia Anti-Rabbit TF AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody(Catalog: MBS2086026))
Western Blot (WB)
(Western Blot;Sample:Lane 1: Rabbit Thymus lysate;Lane 2: Rabbit Testis lysate;Lane 3: Rabbit Lung lysatePrimary Ab: 2ug/ml Cavia Anti-Rabbit TF AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody(Catalog: MBS2086026))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Rabbit Pancreas Tissue;Primary Ab: 20ug/ml Cavia Anti-Rabbit TF AntibodySecond Ab: 2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: MBS2086026))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Rabbit Pancreas Tissue;Primary Ab: 20ug/ml Cavia Anti-Rabbit TF AntibodySecond Ab: 2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: MBS2086026))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tissue factor
NCBI Official Symbol
F3
NCBI Protein Information
tissue factor
UniProt Protein Name
Tissue factor
Protein Family
UniProt Gene Name
F3
UniProt Synonym Gene Names
TF
Uniprot Description
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Research Articles on TF
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Product Notes
The TF f3 (Catalog #AAA2006547) is an Antibody produced from Guinea pig and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tissue Factor (TF) reacts with Oryctolagus cuniculus (Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's Tissue Factor (TF) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TF f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tissue Factor (TF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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