Rabbit anti-Human TIPIN Polyclonal Antibody | anti-TIPIN antibody

Anti-TIPIN Antibody, Rabbit Polyclonal

Cat. Num. AAA8101261

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: TIPIN; Polyclonal Antibody; Anti-TIPIN Antibody; Rabbit Polyclonal; TIPIN Antibody; Rabbit PAb; Antigen Affinity Purified; TIMELESS interacting protein; anti-TIPIN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TIPIN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-TIPIN antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human TIPIN
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TIPIN, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TIPIN rabbit polyclonal antibody at 1:500 dilution Lane A: HCT116 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:50 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of TIPIN in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TIPIN polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)

Immunoprecipitation (IP)

(TIPIN was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti-TIPIN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TIPIN rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :53 kDa)

Related Product Information for anti-TIPIN antibody

Tipin was originally isolated as a protein interacting with Timeless/Tim1/Tim (Tim), which is known to be involved in both circadian rhythm and cell cycle checkpoint regulation. Tim and Tipin form a stable heterodimeric complex that influences checkpoint responses and replication fork progression. The endogenous Tim and Tipin proteins in human cells, interacting through the N-terminal segment of each molecule, form a complex throughout the cell cycle. Tipin and Tim are expressed in the interphase nuclei mostly at constant levels during the cell cycle, and small fractions are recovered in the chromatin-enriched fractions during the S phase. Depletion of endogenous Tipin results in a reduced growth rate, and this may be due in part to inefficient progression of S phase and DNA synthesis. Mammalian Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin in response to replication checkpoint.

References

Yoshizawa-Sugata N, et al. (2007) Human tim/timeless-interacting protein, tipin, is required for efficient progression of s phase and DNA replication checkpoint. J Biol Chem 282 (4): 2729-2740.Errico A, et al. (2009) Tipin/tim1/and1 protein complex promotes pol alpha chromatin binding and sister chromatid cohesion. Embo j 28 (23): 3681-3692.Chou DM, et al. (2006) Tipin and timeless form a mutually protective complex required for genotoxic stress resistance and checkpoint function. Proc Natl Acad Sci U S A 103 (48): 18143-18147.Cho WH, et al. (2013) Human tim-tipin complex affects the biochemical properties of the replicative DNA helicase and DNA polymerases. Proc Natl Acad Sci U S A 110 (7): 2523-2527.

NCBI and Uniprot Product Information

• NCBI GI #: 584613506
• NCBI GeneID: 54962
• NCBI Accession #: NP_001276915.1
• NCBI GenBank Nucleotide #: NM_001289986.1
• UniProt Accession #: Q9BVW5; Q9NWZ6; B2CW64
• Molecular Weight: 34,555 Da
• NCBI Official Full Name: TIMELESS-interacting protein isoform 2
• NCBI Official Synonym Full Names: TIMELESS interacting protein
• NCBI Official Symbol: TIPIN
• NCBI Protein Information: TIMELESS-interacting protein; CSM3 homolog
• UniProt Protein Name: TIMELESS-interacting protein
• Protein Family: TIMELESS-interacting protein
• UniProt Gene Name: TIPIN
• UniProt Entry Name: TIPIN_HUMAN

NCBI Description

The protein encoded by this gene is part of the replisome complex, a group of proteins that support DNA replication. It binds TIM, which is involved in circadian rhythm regulation, and aids in protecting cells against DNA damage and stress. Two pseudogenes and two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

TIPIN: Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Belongs to the CSM3 family.

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: mitosis; intra-S DNA damage checkpoint; cell division; positive regulation of cell proliferation; replication fork protection; DNA replication checkpoint; regulation of DNA replication during S phase; response to UV