Rabbit TIMM50 Polyclonal Antibody | anti-TIMM50 antibody

TIMM50 Antibody

Cat. Num. AAA9612283

• Gene Names: TIMM50; MGCA9; TIM50; TIM50L
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TIMM50; Polyclonal Antibody; TIMM50 Antibody; Homolog of yeast Tim50; Import inner membrane translocase subunit TIM 50; Import inner membrane translocase subunit TIM50; Import inner membrane translocase subunit TIM50 mitochondrial; MGC102733; Mitochondrial import inner membrane translocase subunit TIM50; TIM 50; TIM 50L; TIM50; Tim50 like protein; TIM50_HUMAN; TIM50L; TIMM 50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); Translocase of inner mitochondrial membrane 50 homolog (yeast); Translocase of inner mitochondrial membrane 50 homolog; Translocase of inner mitochondrial membrane 50; yeast; homolog of; anti-TIMM50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIMM50 Antibody detects endogenous levels of total TIMM50.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 456

• Applicable Applications for anti-TIMM50 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TIMM50, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A549, using TIMM50 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-TIMM50 antibody

Function: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.May participate in the release of snRNPs and SMN from the Cajal body.

Subcellular Location: Mitochondrion inner membrane>Single-pass membrane protein. Nucleus speckle. Note: Nuclear and enriched in speckles with snRNPs.

Tissue Specificity: Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).

Subunit Structure: Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23 (PubMed:15044455). The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15 (PubMed:23263864).Interacts with COIL and snRNPs (PubMed:16008839).

Similarity: The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.Belongs to the TIM50 family.

NCBI and Uniprot Product Information

• NCBI GI #: 48526509
• NCBI GeneID: 92609
• NCBI Accession #: NP_001001563.1
• NCBI GenBank Nucleotide #: NP_001001563.1
• UniProt Accession #: Q3ZCQ8
• Molecular Weight: Observed Molecular Weight: (Observed)39kD.; Predicted Molecular Weight: (Calculated)40kDa.
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit TIM50 isoform 1
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 50
• NCBI Official Symbol: TIMM50
• NCBI Official Synonym Symbols: MGCA9; TIM50; TIM50L
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit TIM50
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit TIM50
• Protein Family: Mitochondrial import inner membrane translocase
• UniProt Gene Name: TIMM50
• UniProt Synonym Gene Names: TIM50
• UniProt Entry Name: TIM50_HUMAN

NCBI Description

This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016]