Goat TIM50 Polyclonal Antibody | anti-TIMM50 antibody

Goat anti-TIM50 Antibody

Cat. Num. AAA420718

• Gene Names: TIMM50; TIM50; TIM50L
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TIM50; Polyclonal Antibody; Goat anti-TIM50 Antibody; TIMM50; TIM50L; translocase of inner mitochondrial membrane 50 homolog (yeast); Tim50-like protein; homolog of yeast Tim50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); MGC102733; TIMM50 antibody; TIM50L antibody; translocase of inner mitochondrial membrane 50 homolog (yeast) antibody; Tim50-like protein antibody; homolog of yeast Tim50 antibody; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody; MGC102733 antibody; TIM50 antibody; anti-TIMM50 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CDVRTVLEHYALEDD
• Sequence Length: 456

• Applicable Applications for anti-TIMM50 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Experiments gave bands at approx 40kDa in Human Heart lysate after 0.5ug/ml antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5kDa according to NP_001001563. Recommended concentration: 0.5-3ug/ml.
• Immunogen: Peptide with sequence CDVRTVLEHYALEDD, from the internal region of the protein sequence according to NP_001001563.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (0.5ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 48526509
• NCBI GeneID: 92609
• NCBI Accession #: NP_001001563.1
• NCBI GenBank Nucleotide #: NM_001001563.2
• Molecular Weight: 27,579 Da
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit TIM50
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 50
• NCBI Official Symbol: TIMM50
• NCBI Official Synonym Symbols: TIM50; TIM50L
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit TIM50
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit TIM50
• Protein Family: Mitochondrial import inner membrane translocase
• UniProt Gene Name: TIMM50
• UniProt Synonym Gene Names: TIM50
• UniProt Entry Name: TIM50_HUMAN

Uniprot Description

TIMM50: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. Belongs to the TIM50 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrial inner membrane presequence translocase complex; mitochondrion; nuclear speck; nucleoplasm

Molecular Function: interleukin-2 receptor binding; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; ribonucleoprotein binding; RNA binding

Biological Process: mitochondrial membrane organization and biogenesis; protein amino acid dephosphorylation; protein import into mitochondrial matrix; release of cytochrome c from mitochondria