Western Blot (WB)
(Anti-HAVCR2 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse spleen tissue lysateLane B: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:35 kDa)
Rabbit anti-Mouse TIM-3 Polyclonal Antibody | anti-HAVCR2 antibody
Anti-TIM-3 Antibody, Rabbit Polyclonal
Gene Names
Havcr2; Tim3; TIM-3; Timd3
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
TIM-3; Polyclonal Antibody; Anti-TIM-3 Antibody; Rabbit Polyclonal; TIM-3/HAVCR2 Antibody; Rabbit PAb; Antigen Affinity Purified; hepatitis A virus cellular receptor 2; Anti-Tim3 Antibody; Anti-Timd3 Antibody; anti-HAVCR2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse TIM-3/HAVCR2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HAVCR2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse TIM-3/HAVCR2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse TIM-3/HAVCR2 (rM TIM-3/HAVCR2). TIM-3/HAVCR2 specific IgG was purified by Mouse TIM-3/HAVCR2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-HAVCR2 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse spleen tissue lysateLane B: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:35 kDa)
Western Blot (WB)
(Anti-HAVCR2 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse spleen tissue lysateLane B: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:35 kDa)
Related Product Information for anti-HAVCR2 antibody
Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
Product Categories/Family for anti-HAVCR2 antibody
References
Geng H, et al. (2006) Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3): 1411-20.Anderson AC, et al. (2006) TIM-3 in autoimmunity. Curr Opin Immunol. 18(6): 665-9.Anderson DE. (2007) TIM-3 as a therapeutic target in human inflammatory diseases. Expert Opin Ther Targets. 11(8): 1005-9.Pan HF, et al. (2010) TIM-3 as a new therapeutic target in systemic lupus erythematosus. Mol Biol Rep. 37(1): 395-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,087 Da
NCBI Official Full Name
T cell immunoglobulin mucin-3
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
Havcr2
NCBI Official Synonym Symbols
Tim3; TIM-3; Timd3
NCBI Protein Information
hepatitis A virus cellular receptor 2 homolog
Protein Family
Research Articles on HAVCR2
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Product Notes
The HAVCR2 (Catalog #AAA8112370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIM-3 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the HAVCR2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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