TIM-3 / HAVCR2 recombinant protein

Recombinant Mouse TIM-3 / HAVCR2 Protein (His tag)

Cat. Num. AAA2546641

• Gene Names: Havcr2; Tim3; TIM-3; Timd3
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TIM-3 / HAVCR2; Recombinant Mouse TIM-3 / HAVCR2 Protein (His tag); HAVCR2; TIM-3 / HAVCR2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence Length: 281

• Application Notes: The recombinant mouse HAVCR2 comprises 181 amino acids and has a predicted molecular mass of 20.3 kDa. The apparent molecular mass of the protein is approximately 37-47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Leu 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TIM-3 / HAVCR2 recombinant protein

Background: Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In tumor rejection model, soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.

Description: A DNA sequence encoding the mouse HAVCR2 (AAL65156.1) (Met1-Arg191) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 18182531
• NCBI GeneID: 171285
• Molecular Weight: 22,087 Da
• NCBI Official Full Name: T cell immunoglobulin mucin-3
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 2
• NCBI Official Symbol: Havcr2
• NCBI Official Synonym Symbols: Tim3; TIM-3; Timd3
• NCBI Protein Information: hepatitis A virus cellular receptor 2 homolog
• UniProt Protein Name: Hepatitis A virus cellular receptor 2 homolog
• UniProt Gene Name: Havcr2
• UniProt Synonym Gene Names: Tim3; Timd3; HAVcr-2; TIMD-3; TIM-3

Uniprot Description

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11|11 B1.1

Cellular Component: cell surface; early endosome; immunological synapse

Molecular Function: protein binding

Biological Process: defense response to Gram-positive bacterium; inhibition of NF-kappaB transcription factor; macrophage activation during immune response; maternal process involved in pregnancy; natural killer cell tolerance induction; negative regulation of immune response to tumor cell; negative regulation of innate immune response; negative regulation of interferon type I production; negative regulation of interferon-alpha production; negative regulation of interferon-gamma production; negative regulation of interleukin-2 production; negative regulation of interleukin-3 production; negative regulation of interleukin-6 production; negative regulation of myeloid dendritic cell activation; negative regulation of natural killer cell activation; negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of T cell proliferation; negative regulation of T-helper 1 type immune response; negative regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of innate immune response; positive regulation of interferon-gamma production; positive regulation of interleukin-1 production; positive regulation of interleukin-4 production; positive regulation of macrophage activation; positive regulation of T cell proliferation; regulation of tolerance induction dependent upon immune response; toll-like receptor 3 signaling pathway; toll-like receptor 7 signaling pathway; toll-like receptor 9 signaling pathway