Testing Data
TIM-3 ELISA Pair Set | HAVCR2 elisa pair set
TIM-3 Matched ELISA Antibody Pair Set, Human
Synonyms
TIM-3; TIM-3 Matched ELISA Antibody Pair Set; Human; Human TIM-3/HAVCR2 ELISA Pair Set; hepatitis A virus cellular receptor 2; CD366 Matched ELISA Antibody Pair Set; HAVcr-2 Matched ELISA Antibody Pair Set; HAVCR2 Matched ELISA Antibody Pair Set; KIM-3 Matched ELISA Antibody Pair Set; Tim-3 Matched ELISA Antibody Pair Set; TIM3 Matched ELISA Antibody Pair Set; TIMD-3 Matched ELISA Antibody Pair Set; TIMD3 Matched ELISA Antibody Pair Set; HAVCR2 elisa pair set
Specificity
Quantitative determination of Human TIM-3
Assay Type
Solid Phase Sandwich ELISA
Detection Range
31.25-2000pg/mL
Sensitivity
31.25pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human TIM-3/HAVCR2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TIM-3/HAVCR2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 85 ng of recombinant Human TIM-3/HAVCR2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TIM-3/HAVCR2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 85 ng of recombinant Human TIM-3/HAVCR2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for HAVCR2 elisa pair set
Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
This TIM-3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human TIM-3. It contains Human TIM-3 capture antibody, Human TIM-3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human TIM-3 protein.
This TIM-3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human TIM-3. It contains Human TIM-3 capture antibody, Human TIM-3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human TIM-3 protein.
Product Categories/Family for HAVCR2 elisa pair set
References
Geng H, et al. (2006) Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3): 1411-20.Anderson AC, et al. (2006) TIM-3 in autoimmunity. Curr Opin Immunol. 18(6): 665-9.Anderson DE. (2007) TIM-3 as a therapeutic target in human inflammatory diseases. Expert Opin Ther Targets. 11(8): 1005-9.Pan HF, et al. (2010) TIM-3 as a new therapeutic target in systemic lupus erythematosus. Mol Biol Rep. 37(1): 395-8.
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Product Notes
The HAVCR2 (Catalog #AAA8118242) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TIM-3, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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