Rabbit TIEG2 Polyclonal Antibody | anti-TIEG2 antibody

TIEG2 (F6) Polyclonal Antibody

Cat. Num. AAA3001796

• Gene Names: KLF11; FKLF; FKLF1; MODY7; TIEG2; Tieg3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TIEG2; Polyclonal Antibody; TIEG2 (F6) Polyclonal Antibody; Krueppel-like factor 11; Transforming growth factor-beta-inducible early growth response protein 2; TGFB-inducible early growth response protein 2; TIEG-2; KLF11; FKLF; anti-TIEG2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIEG2 (F6) polyclonal antibody detects endogenous levels of TIEG2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 495

• Applicable Applications for anti-TIEG2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human TIEG2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TIEG2 (F6) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:The Heart tissue lysate of Mouse (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TIEG2 (F6) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-TIEG2 antibody

TIEG1 and EGR-alpha are expressedfrom alternate promoters of the same gene. TIEG1 and EGR-alpha are both highly expressed in human fetal osteoblast cells. TIEG1 is additionally expressed at high levels in PBLs, spleen and colon, and at lower levels in thymus, small intestine, ovary, prostate and skeletal muscle. The nuclear TIEG2 protein, which shares significant homology with TIEG1, was originally isolated from globin-expressing human fetal eryth-roid cells. TIEG2 is also expressed in fetal liver. Overexpression of TIEG2 in cultured epithelial cells inhibits cellular proliferation; TIEG2 expression is upregulated by TGFbeta1 and serum deprivation.

NCBI and Uniprot Product Information

• NCBI GI #: 295148161
• NCBI GeneID: 8462
• NCBI Accession #: NP_001171187.1
• NCBI GenBank Nucleotide #: NP_001171187.1
• UniProt Accession #: O14901
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: Krueppel-like factor 11 isoform b
• NCBI Official Synonym Full Names: Kruppel like factor 11
• NCBI Official Symbol: KLF11
• NCBI Official Synonym Symbols: FKLF; FKLF1; MODY7; TIEG2; Tieg3
• NCBI Protein Information: Krueppel-like factor 11
• UniProt Protein Name: Krueppel-like factor 11
• UniProt Gene Name: KLF11
• UniProt Synonym Gene Names: FKLF; TIEG2; TGFB-inducible early growth response protein 2; TIEG-2
• UniProt Entry Name: KLF11_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

TIEG2: Transcription factor. Activates the epsilon- and gamma- globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis. Defects in KLF11 are the cause of maturity-onset diabetes of the young type 7 (MODY7). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; G1/S-specific transcription in mitotic cell cycle; negative regulation of cell proliferation; positive regulation of apoptosis; apoptosis; negative regulation of transcription from RNA polymerase II promoter

Disease: Maturity-onset Diabetes Of The Young, Type 7