Goat anti-Human TICAM1 Polyclonal Antibody | anti-TICAM1 antibody

TICAM1 (TIR Domain-containing Adapter Molecule 1, TICAM-1, Proline-rich, Vinculin and TIR Domain-containing Protein B, Putative NF-kappa-B-activating Protein 502H, Toll-interleukin-1 Receptor Domain-containing Adapter Protein Inducing Interferon beta, TIR

Cat. Num. AAA626029

• Gene Names: TICAM1; TRIF; PRVTIRB; TICAM-1; MGC35334
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TICAM1; Polyclonal Antibody; TICAM1 (TIR Domain-containing Adapter Molecule 1; TICAM-1; Proline-rich; Vinculin and TIR Domain-containing Protein B; Putative NF-kappa-B-activating Protein 502H; Toll-interleukin-1 Receptor Domain-containing Adapter Protein Inducing Interferon beta; TIR; Anti -TICAM1 (TIR Domain-containing Adapter Molecule 1; anti-TICAM1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIF. Species sequence homology: mouse (62%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.

• Applicable Applications for anti-TICAM1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:0.25ug/ml
• Immunogen: Purified, E. coli-derived, recombinant human TIR domain-containing adaptor inducing IFN-b (aa29-204; Accession #Q8IUC6).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TICAM1 antibody

TRIF (TIR domain-containing adaptor inducing IFN-b; also TICAM1) is a 105-110kD cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712aa in length and contains three TRAF6 bonding motifs (aa84-91, 248-255 and 299-309), one TIR domain (aa390-460), a Pro-rich region (aa614-678), and an overlapping RHIM domain (aa661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23aa substitution for aa31-162 accompanied by a Pro substitution for aa633-660, a second shows a 44aa substitution for aa352-712, and a third shows a 38aa substitution for aa271-712. Over aa29-204, human TRIF shares 62% aa identity with mouse TRIF.

Product Categories/Family for anti-TICAM1 antibody

Antibodies; Abs to Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 41281981
• NCBI GeneID: 148022
• NCBI Accession #: NP_891549.1
• NCBI GenBank Nucleotide #: NM_182919.2
• UniProt Accession #: Q8IUC6; O75532; Q86XP8; Q96GA0; B3Y691
• Molecular Weight: 76,422 Da
• NCBI Official Full Name: TIR domain-containing adapter molecule 1
• NCBI Official Synonym Full Names: toll-like receptor adaptor molecule 1
• NCBI Official Symbol: TICAM1
• NCBI Official Synonym Symbols: TRIF; PRVTIRB; TICAM-1; MGC35334
• NCBI Protein Information: TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; putative NF-kappa-B-activating protein 502H; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter protein inducing IFN-beta; proline-rich, vinculin and TIR domain-containing protein B; toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
• UniProt Protein Name: TIR domain-containing adapter molecule 1
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM1
• UniProt Synonym Gene Names: PRVTIRB; TRIF
• UniProt Entry Name: TCAM1_HUMAN

NCBI Description

TRIF, or TICAM1, is a Toll/IL1R (MIM 147810) (TIR) domain-containing adaptor molecule, like MYD88 (MIM 602170) and TIRAP (MIM 606252), that induces interferon-beta (IFNB; MIM 147640), specifically interacts with TLR3 (MIM 603029), and activates nuclear factor kappa-B (NFKB; see MIM 164011).[supplied by OMIM]

Uniprot Description

TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endosome membrane; cytosol

Molecular Function: signal transducer activity; protein binding; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; MyD88-independent toll-like receptor signaling pathway; regulation of protein homodimerization activity; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; response to exogenous dsRNA; positive regulation of chemokine biosynthetic process; positive regulation of protein ubiquitination; positive regulation of interferon-beta biosynthetic process; macrophage activation during immune response; positive regulation of B cell proliferation; toll-like receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway

Disease: Herpes Simplex Encephalitis, Susceptibility To, 4