Sheep Thymidylate Synthase Polyclonal Antibody | anti-Tyms antibody

Anti-Human Thymidylate Synthase Antibody

Cat. Num. AAA4157852

• Gene Names: Tyms; Ts
• Reactivity: Human, Mouse Rat
• Applications: Western Blot, Immunoprecipitation, ELISA
• Purity: Affinity Purified
• Synonyms: Thymidylate Synthase; Polyclonal Antibody; Anti-Human Thymidylate Synthase Antibody; Human Thymidylate Synthase; anti-Tyms antibody

• Host: Sheep
• Reactivity: Human, Mouse Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This purified IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 307

• Applicable Applications for anti-Tyms antibody: Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: This purified IgG fraction against Human Thymidylate Synthase (hTS) has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMPreduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
• Immunogen: This antibody was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 46358062
• NCBI GeneID: 22171
• NCBI GenBank Nucleotide #: NP_067263.1
• UniProt Accession #: P07607
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthase
• NCBI Official Symbol: Tyms
• NCBI Official Synonym Symbols: Ts
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• Protein Family: Thymidylate synthase
• UniProt Gene Name: Tyms
• UniProt Synonym Gene Names: TS; TSase

NCBI Description

This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010]

Uniprot Description

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.