Mouse anti-Human Thymidylate Synthase Monoclonal Antibody | anti-TYMS antibody

Thymidylate Synthase (5-FU Resistance Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438476

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Thymidylate Synthase; Monoclonal Antibody; Thymidylate Synthase (5-FU Resistance Marker) Mouse Monoclonal Antibody; dTMP synthase; TMS; TS; TSase; TYMS protein; Tyms thymidylate synthetase; anti-TYMS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: TMS715
• Specificity: It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 313

• Applicable Applications for anti-TYMS antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclear & Cytoplasmic
• Immunogen: Recombinant human thymidylate synthase
• Hu-Chromosome Location: 18p11.32
• Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody is stable for 24 months.,Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Monoclonal Antibody (TMS715).)

References

Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31

NCBI and Uniprot Product Information

• NCBI GI #: 4507751
• NCBI GeneID: 7298
• NCBI Accession #: NP_001062.1
• NCBI GenBank Nucleotide #: NM_001071.2
• UniProt Accession #: P04818; Q8WYK3; Q8WYK4
• Molecular Weight: 36kDa
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• Protein Family: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase
• UniProt Entry Name: TYSY_HUMAN

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

Uniprot Description

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Mitochondrial; EC 2.1.1.45; Nucleotide Metabolism - pyrimidine; Methyltransferase; DNA repair, damage; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; nucleoplasm; nucleus

Molecular Function: folic acid binding; thymidylate synthase activity

Biological Process: deoxyribonucleoside monophosphate biosynthetic process; dTMP biosynthetic process; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside biosynthetic process; response to organophosphorus