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Western Blot (WB) (Western blot analysis of Thrombospondin 4 expression in A431 (A), HEK293T (B) whole cell lysates.)

Rabbit anti-Human Thrombospondin 4 Polyclonal Antibody | anti-THBS4 antibody

Anti-Thrombospondin 4 Antibody

Gene Names
THBS4; TSP4; TSP-4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
Thrombospondin 4; Polyclonal Antibody; Anti-Thrombospondin 4 Antibody; TSP4; Thrombospondin-4; anti-THBS4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Thrombospondin 4 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
961
Applicable Applications for anti-THBS4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Thrombospondin 4. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Thrombospondin 4 expression in A431 (A), HEK293T (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of Thrombospondin 4 expression in A431 (A), HEK293T (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Thrombospondin 4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of Thrombospondin 4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-THBS4 antibody
Rabbit polyclonal antibody to Thrombospondin 4

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Thrombospondin-4
NCBI Official Synonym Full Names
thrombospondin 4
NCBI Official Symbol
THBS4
NCBI Official Synonym Symbols
TSP4; TSP-4
NCBI Protein Information
thrombospondin-4
UniProt Protein Name
Thrombospondin-4
Protein Family
UniProt Gene Name
THBS4
UniProt Synonym Gene Names
TSP4
UniProt Entry Name
TSP4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. It is involved in local signaling in the developing and adult nervous system, and it contributes to spinal sensitization and neuropathic pain states. This gene is activated during the stromal response to invasive breast cancer. It may also play a role in inflammatory responses in Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

THBS4: Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: basement membrane; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; neuromuscular junction; sarcoplasmic reticulum

Molecular Function: calcium ion binding; collagen binding; fibronectin binding; growth factor activity; heparin binding; integrin binding; laminin-1 binding; protein binding

Biological Process: behavioral response to pain; myoblast migration; negative regulation of angiogenesis; neurite morphogenesis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; protein homooligomerization; regulation of tissue remodeling; response to unfolded protein; tissue remodeling

Research Articles on THBS4

Similar Products

Product Notes

The THBS4 thbs4 (Catalog #AAA8241709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Thrombospondin 4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thrombospondin 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the THBS4 thbs4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombospondin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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