Human Thrombospondin-4 ELISA Kit | THBS4 elisa kit

Human Thrombospondin-4 ELISA Kit

Cat. Num. AAA760986

• Gene Names: THBS4; TSP4; TSP-4
• Reactivity: Human
• Synonyms: Thrombospondin-4; Human Thrombospondin-4 ELISA Kit; TSP-4 (Thrombospondin-4)/THBS4; THBS4 elisa kit

• Reactivity: Human
• Sequence Length: 961

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for THBS4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TSP-4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TSP-4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TSP-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TSP-4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31543806
• NCBI GeneID: 7060
• NCBI Accession #: NP_003239.2
• NCBI GenBank Nucleotide #: NM_003248.5
• Molecular Weight: 105,869 Da
• NCBI Official Full Name: thrombospondin-4 isoform a
• NCBI Official Synonym Full Names: thrombospondin 4
• NCBI Official Symbol: THBS4
• NCBI Official Synonym Symbols: TSP4; TSP-4
• NCBI Protein Information: thrombospondin-4
• UniProt Protein Name: Thrombospondin-4
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS4
• UniProt Synonym Gene Names: TSP4
• UniProt Entry Name: TSP4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. It is involved in local signaling in the developing and adult nervous system, and it contributes to spinal sensitization and neuropathic pain states. This gene is activated during the stromal response to invasive breast cancer. It may also play a role in inflammatory responses in Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

THBS4: Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: basement membrane; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; neuromuscular junction; sarcoplasmic reticulum

Molecular Function: calcium ion binding; collagen binding; fibronectin binding; growth factor activity; heparin binding; integrin binding; laminin-1 binding; protein binding

Biological Process: behavioral response to pain; myoblast migration; negative regulation of angiogenesis; neurite morphogenesis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; protein homooligomerization; regulation of tissue remodeling; response to unfolded protein; tissue remodeling