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Testing Data

Mouse anti-Mouse NK1.1 Cell Surface Antigen Monoclonal Antibody

NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c)

Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Purified
Purified
Synonyms
NK1.1 Cell Surface Antigen; Monoclonal Antibody; NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C; CD161 Antigen-like Family Member C; Lymphocyte Antigen 55c; Ly-55c; NKR-P1.9; NKR-P1C; Natural Killer Cell Surface Protein P1-40; NKR-P1 40; CD161c; Klrb1c; Ly55c; Nkrp1c); Anti -NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C; anti-NK1.1 Cell Surface Antigen antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
PK136
Specificity
Recognizes mouse NK1.1 alloantigen, Mr 80-85kD (39kD under reducing conditions).
Purity/Purification
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
Applicable Applications for anti-NK1.1 Cell Surface Antigen antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Flow cytometry 1, , In vitro complement-dependent cytotoxicity assays 1, , Immunoprecipitation 8, , In vivo depletion and in vitro activation of NK1.1 cells 5,6, , In vitro redirected lysis 5
Dilution: Flow Cytometry: 1ug/10e6 cells
Immunogen
CD mouse spleen and bone marrow cells enriched for NK-1+ cells
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Testing Data
Related Product Information for anti-NK1.1 Cell Surface Antigen antibody
NK1.1, a member of the NKR-PI family of cell surface receptors, is a type II integral membrane protein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57/BL6, FVB/N, NZB; but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, SJL, 129).1-4 NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells.5-7
Product Categories/Family for anti-NK1.1 Cell Surface Antigen antibody

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Product Notes

The NK1.1 Cell Surface Antigen (Catalog #AAA602883) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NK1.1 Cell Surface Antigen can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Flow cytometry 1,, In vitro complement-dependent cytotoxicity assays 1,, Immunoprecipitation 8,, In vivo depletion and in vitro activation of NK1.1 cells 5,6,, In vitro redirected lysis 5 Dilution: Flow Cytometry: 1ug/10e6 cells. Researchers should empirically determine the suitability of the NK1.1 Cell Surface Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NK1.1 Cell Surface Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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