Rabbit TH Polyclonal Antibody | anti-TH antibody

TH (G13) Polyclonal Antibody

Cat. Num. AAA3000945

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TH; Polyclonal Antibody; TH (G13) Polyclonal Antibody; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TYH; anti-TH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Tyrosine Hydroxylase (G13) polyclonal antibody detects endogenous levels of Tyrosine Hydroxylase protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 497

• Applicable Applications for anti-TH antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human TH.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TH (G13) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-TH antibody

The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson's disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds

NCBI and Uniprot Product Information

• NCBI GI #: 88900503
• NCBI GeneID: 7054
• NCBI Accession #: NP_000351.2
• NCBI GenBank Nucleotide #: NP_000351.2
• UniProt Accession #: P07101
• Molecular Weight: ~ 58kDa
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform b
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• Protein Family: THP3
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Oxidoreductase; Endoplasmic reticulum; Mitochondrial; Vesicle; Amino Acid Metabolism - tyrosine; EC 1.14.16.2

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; response to zinc ion; cerebral cortex development; response to activity; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to salt stress; response to estradiol stimulus; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive