Rabbit anti-Human, Mouse TFE3 Polyclonal Antibody | anti-TFE3 antibody

TFE3, CT (TFE3, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33) (PE)

Cat. Num. AAA6355051

• Gene Names: TFE3; TFEA; RCCP2; RCCX1; bHLHe33
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TFE3; Polyclonal Antibody; CT (TFE3; BHLHE33; Transcription factor E3; Class E basic helix-loop-helix protein 33) (PE); Class E basic helix-loop-helix protein 33; anti-TFE3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TFE3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 488-516 amino acids from the C-terminal region of human TFE3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TFE3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).)

Product Categories/Family for anti-TFE3 antibody

Antibodies; Transcription Factors

References

Argani, P., et al. Am. J. Surg. Pathol. 34(10):1395-1406(2010). Haudebourg, J., et al. Cancer Genet. Cytogenet. 200(2):75-78(2010). Chang, I.W., et al. Am. J. Surg. Pathol. 33(12):1894-1901(2009). Yamaguchi, T., et al. Acta Cytol. 53(6):693-697(2009). Kuroda, N., et al. Pathol. Int. 59(10):769-770(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 8659574
• NCBI GeneID: 7030
• NCBI Accession #: NP_006512
• NCBI GenBank Nucleotide #: NM_006521
• UniProt Accession #: P19532; Q92757; Q92758; Q99964; A8MZL6
• Molecular Weight: 61,521 Da
• NCBI Official Full Name: transcription factor E3
• NCBI Official Synonym Full Names: transcription factor binding to IGHM enhancer 3
• NCBI Official Symbol: TFE3
• NCBI Official Synonym Symbols: TFEA; RCCP2; RCCX1; bHLHe33
• NCBI Protein Information: transcription factor E3; Transcription factor for IgH enhancer; transcription factor E family, member A; class E basic helix-loop-helix protein 33
• UniProt Protein Name: Transcription factor E3
• Protein Family: Transcription factor
• UniProt Gene Name: TFE3
• UniProt Synonym Gene Names: BHLHE33; bHLHe33
• UniProt Entry Name: TFE3_HUMAN

Uniprot Description

TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: regulation of osteoclast differentiation; positive regulation of cell adhesion; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; humoral immune response

Disease: Renal Cell Carcinoma, Xp11-associated