Rabbit anti-Human TFAP2B Polyclonal Antibody | anti-TFAP2B antibody

TFAP2B Polyclonal Antibody

Cat. Num. AAA9127969

• Gene Names: TFAP2B; AP-2B; AP2-B
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: TFAP2B; Polyclonal Antibody; TFAP2B Polyclonal Antibody; anti-TFAP2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 469

• Applicable Applications for anti-TFAP2B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:500 - 1:2000; IHC 1:50 - 1:200
• Route: Synthetic Peptide
• Immunogen: A synthetic peptide of human TFAP2B
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TFAP2B antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Related Product Information for anti-TFAP2B antibody

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives.

NCBI and Uniprot Product Information

• NCBI GI #: 118582289
• NCBI GeneID: 7021
• NCBI Accession #: NP_003212.2
• NCBI GenBank Nucleotide #: NM_003221.3
• UniProt Accession #: Q92481; Q5JYX6; Q9NQ63; Q9NU99; Q9UJI7; Q9Y214; Q9Y3K3
• Molecular Weight: 50kDa
• NCBI Official Full Name: transcription factor AP-2-beta
• NCBI Official Synonym Full Names: transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
• NCBI Official Symbol: TFAP2B
• NCBI Official Synonym Symbols: AP-2B; AP2-B
• NCBI Protein Information: transcription factor AP-2-beta
• UniProt Protein Name: Transcription factor AP-2-beta
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2B
• UniProt Synonym Gene Names: AP2-beta
• UniProt Entry Name: AP2B_HUMAN

NCBI Description

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq, Jul 2008]

Uniprot Description

AP-2 beta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Belongs to the AP-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; caspase inhibitor activity; chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; skin development; hindlimb morphogenesis; positive regulation of transcription, DNA-dependent; response to lithium ion; regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; glucose homeostasis; calcium ion homeostasis; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; regulation of cell differentiation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; sodium ion homeostasis; negative regulation of neuron apoptosis; phosphate ion homeostasis; kidney development; potassium ion homeostasis; response to drug; glucose metabolic process; renal water homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; negative regulation of apoptosis

Disease: Char Syndrome