Rabbit TFAM Polyclonal Antibody | anti-TFAM antibody

TFAM Polyclonal Antibody

Cat. Num. AAA3007352

• Gene Names: TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3; MTDPS15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TFAM; Polyclonal Antibody; TFAM Polyclonal Antibody; Transcription factor A; mitochondrial; mtTFA; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2; TCF6; TCF6L2; anti-TFAM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFAM polyclonal antibody detects endogenous levels of TFAM protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 246

• Applicable Applications for anti-TFAM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 126-171 of Human TFAM.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TFAM polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:RAW264. 7 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug))

Related Product Information for anti-TFAM antibody

mtTFA (mitochondrial transcription factor A), also known as mtTF1, TFAM, TCF6 (for transcription factor 6-like1), TCF6L2 and tsHMG, is a nuclear-encoded gene product that is imported into the mitochondria. mtTFA is required for many aspects of mitochondrial biogenesis including the replication and transcription of mitochondrial DNA (mtDNA). In mouse, testis-specific mtTFA is missing the mitochondria targeting sequence and is present in the nucleus rather than the mitochondria. This form of mtTFA is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. During mouse and human spermatogenesis there is a reduction of mtTFA protein levels and a reduction in mtDNA copy number. These features may provide one of the mechanisms by which paternal mtDNA transmission is prevented. mtTFA has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.

NCBI and Uniprot Product Information

• NCBI GI #: 4507401
• NCBI GeneID: 7019
• NCBI Accession #: NP_003192.1
• NCBI GenBank Nucleotide #: NP_003192.1
• UniProt Accession #: Q00059
• Molecular Weight: ~ 26kDa
• NCBI Official Full Name: transcription factor A, mitochondrial isoform 1
• NCBI Official Synonym Full Names: transcription factor A, mitochondrial
• NCBI Official Symbol: TFAM
• NCBI Official Synonym Symbols: TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3; MTDPS15
• NCBI Protein Information: transcription factor A, mitochondrial
• UniProt Protein Name: Transcription factor A, mitochondrial
• Protein Family: Transcription factor
• UniProt Gene Name: TFAM
• UniProt Synonym Gene Names: TCF6; TCF6L2; mtTFA; MtTF1; TCF-6
• UniProt Entry Name: TFAM_HUMAN

NCBI Description

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: mitochondrion; mitochondrial matrix; cytosol; nucleus

Molecular Function: protein binding; heat shock protein binding; chromatin binding; DNA bending activity; transcription factor activity

Biological Process: mitochondrial respiratory chain complex assembly; chromatin remodeling; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter