Western Blot (WB)
(Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.)
Rabbit anti-Human TESPA1 Polyclonal Antibody | anti-TESPA1 antibody
TESPA1 Antibody
Gene Names
TESPA1; HSPC257; KIAA0748
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
TESPA1 antibody is affinity chromatography purified via peptide column.
Synonyms
TESPA1; Polyclonal Antibody; TESPA1 Antibody; HSPC257; KIAA0748; Protein TESPA1; Thymocyte-expressed positive selection-associated protein 1; thymocyte expressed; positive selection associated 1; anti-TESPA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.
Purity/Purification
TESPA1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
521
Applicable Applications for anti-TESPA1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1.
Buffer
TESPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TESPA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.)
Western Blot (WB)
(Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.)
Immunocytochemistry (IHC)
(Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.)
Related Product Information for anti-TESPA1 antibody
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 57 kDa
Observed: 60 kDa
Observed: 60 kDa
NCBI Official Full Name
protein TESPA1 isoform 1
NCBI Official Synonym Full Names
thymocyte expressed, positive selection associated 1
NCBI Official Symbol
TESPA1
NCBI Official Synonym Symbols
HSPC257; KIAA0748
NCBI Protein Information
protein TESPA1; thymocyte-expressed positive selection-associated protein 1
UniProt Protein Name
Protein TESPA1
Protein Family
UniProt Gene Name
TESPA1
UniProt Synonym Gene Names
KIAA0748
UniProt Entry Name
TESP1_HUMAN
Uniprot Description
TESPA1: Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development. Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 12q13.2
Cellular Component: signalosome; endoplasmic reticulum membrane; cytoplasm
Molecular Function: receptor binding
Biological Process: positive regulation of T cell differentiation in the thymus; positive regulation of T cell receptor signaling pathway; signalosome assembly
Research Articles on TESPA1
Similar Products
Product Notes
The TESPA1 tespa1 (Catalog #AAA153352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TESPA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TESPA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the TESPA1 tespa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TESPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
