Rabbit SPRYD4 Polyclonal Antibody | anti-SPRYD4 antibody

SPRYD4 Antibody

Cat. Num. AAA151300

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SPRYD4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SPRYD4; Polyclonal Antibody; SPRYD4 Antibody; SPRY domain-containing protein 4; SPRY domain containing 4; anti-SPRYD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: SPRYD4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-SPRYD4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: SPRYD4 antibody can be used for detection of SPRYD4 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SPRYD4 antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4.
• Buffer: SPRYD4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SPRYD4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SPRYD4 in fetal human liver tissue lysate with SPRYD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide)

Immunohistochemistry (IHC)

(Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 μg/mL.)

Related Product Information for anti-SPRYD4 antibody

SPRYD4 Antibody: The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.

NCBI and Uniprot Product Information

• NCBI GI #: 46409324
• NCBI GeneID: 283377
• NCBI Accession #: NP_997227
• NCBI GenBank Nucleotide #: NM_207344.3
• UniProt Accession #: Q8WW59; A8K7A5
• Molecular Weight: 23,129 Da
• NCBI Official Full Name: SPRY domain-containing protein 4
• NCBI Official Synonym Full Names: SPRY domain containing 4
• NCBI Official Symbol: SPRYD4
• NCBI Protein Information: SPRY domain-containing protein 4
• UniProt Protein Name: SPRY domain-containing protein 4
• Protein Family: SPRY domain-containing protein
• UniProt Gene Name: SPRYD4
• UniProt Entry Name: SPRY4_HUMAN

Uniprot Description

SPRYD4:

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrion; nucleus