Rabbit anti-Human ABCA7 Polyclonal Antibody | anti-ABCA7 antibody

ABCA7 Antibody

Cat. Num. AAA150228

• Gene Names: ABCA7; ABCX; ABCA-SSN
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ABCA7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ABCA7; Polyclonal Antibody; ABCA7 Antibody; ABCX; ABCA-SSN; ATP-binding cassette sub-family A member 7; ATP-binding cassette; sub-family A (ABC1); member 7; anti-ABCA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABCA7 antibody is human specific. At least two alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Purity/Purification: ABCA7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 2146

• Applicable Applications for anti-ABCA7 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ABCA7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ABCA7.
• Buffer: ABCA7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ABCA7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 μg/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 μg/ml.Red: ABCA7 Antibody (6889)Blue: DAPI staining)

Related Product Information for anti-ABCA7 antibody

ABCA7 Antibody: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family that use ATP hydrolysis to catalyze the transport of various molecules across cell membranes. They are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCA7, a member of the ABC1 subfamily, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. ABCA7 shows the highest homology to ABCA1, an essential molecule for cholesterol homeostasis. The high expression levels of ABCA7 in peripheral leukocytes, thymus, spleen and bone marrow suggests a role in the immune system lipid homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 150417984
• NCBI GeneID: 10347
• NCBI Accession #: NP_061985
• NCBI GenBank Nucleotide #: NM_019112.3
• UniProt Accession #: Q8IZY2; Q96S58; Q9BZC4; Q9NR73; Q9UKP8
• Molecular Weight: 218,497 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 7
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 7
• NCBI Official Symbol: ABCA7
• NCBI Official Synonym Symbols: ABCX; ABCA-SSN
• NCBI Protein Information: ATP-binding cassette sub-family A member 7; autoantigen SS-N; macrophage ABC transporter
• UniProt Protein Name: ATP-binding cassette sub-family A member 7
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA7
• UniProt Entry Name: ABCA7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA7: Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Belongs to the ABC transporter superfamily. ABCA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; Golgi apparatus; cell surface; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; ATP-binding cassette (ABC) transporter complex; phagocytic cup

Molecular Function: apolipoprotein A-I receptor activity; phospholipid transporter activity; transporter activity; ATPase activity; ATP binding

Biological Process: phospholipid efflux; positive regulation of ATPase activity; positive regulation of phagocytosis; negative regulation of amyloid precursor protein biosynthetic process; cholesterol efflux; peptide cross-linking; phospholipid scrambling; negative regulation of ATPase activity; transmembrane transport; phagocytosis; memory