Rabbit anti-Human TERT Polyclonal Antibody | anti-TERT antibody
TERT, phosphorylated (Tyr707) (Telomerase Reverse Transcriptase, Telomerase Catalytic Subunit, HEST2, Telomerase-associated Protein 2, TP2, EST2, TCS1, TRT)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Dot Blot: 1:500
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Phospho-TERT-pY707 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-TERT-pY707 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
NCBI and Uniprot Product Information
NCBI Description
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.
Protein type: Nucleolus; Transferase; EC 2.7.7.49; DNA-binding
Chromosomal Location of Human Ortholog: 5p15.33
Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolus
Molecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA binding
Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; telomere maintenance; RNA interference, production of siRNA; positive regulation of Wnt receptor signaling pathway
Disease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1
