Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Tenascin C Polyclonal Antibody | anti-TNC antibody
Polyclonal Antibody to Tenascin C (TNC)
Gene Names
Tnc; TN; Hxb; Ten; TN-C; AI528729; cytotactin; tenascin-C; C130033P17Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Tenascin C; Polyclonal Antibody; Polyclonal Antibody to Tenascin C (TNC); anti-TNC antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TNC. It has beenselected for its ability to recognize TNC in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CVCEPGW KGPNCSEPDC PGNCNLRGQC LDGQCICDEG FTGEDCSQLA CPNDCNDQGR CVNGVCVCFE GYAGPDCGLE VCPVPCSEEH GMCVDGRCVC KDGFAGEDCN EPLCLNNCYN RGRCVENECV CDEGFTGEDC SELICPNDCF DRGRCINGTC YCEEGFTGED CGELTCPNDC QGRGQCEEGQ CVCNEGFAGA DCSEKRCPAD CHHRGRCLNG QCECDDGFTG ADCGDLQCPN GCSGHGRCVN GQCVCDEGYT GEDCSQRRCP NDCHNRGLCV QGKCICEQGF KGFDCSEMSC PNDCHQHGRC VNGMCICDDD YTGEDCRDRR CPRDCSQRGR CVDGQCICED GFTGPDCAEL SCPSDCHGHG RCVNGQCICH EGFTGKDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CVCEPGW KGPNCSEPDC PGNCNLRGQC LDGQCICDEG FTGEDCSQLA CPNDCNDQGR CVNGVCVCFE GYAGPDCGLE VCPVPCSEEH GMCVDGRCVC KDGFAGEDCN EPLCLNNCYN RGRCVENECV CDEGFTGEDC SELICPNDCF DRGRCINGTC YCEEGFTGED CGELTCPNDC QGRGQCEEGQ CVCNEGFAGA DCSEKRCPAD CHHRGRCLNG QCECDDGFTG ADCGDLQCPN GCSGHGRCVN GQCVCDEGYT GEDCSQRRCP NDCHNRGLCV QGKCICEQGF KGFDCSEMSC PNDCHQHGRC VNGMCICDDD YTGEDCRDRR CPRDCSQRGR CVDGQCICED GFTGPDCAEL SCPSDCHGHG RCVNGQCICH EGFTGKDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG
Sequence Length
242
Applicable Applications for anti-TNC antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
TNC (Cys174~Ser621)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant TNC (Cys174~Ser621) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061397
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
172,354 Da
NCBI Official Full Name
tenascin
NCBI Official Synonym Full Names
tenascin C
NCBI Official Symbol
Tnc
NCBI Official Synonym Symbols
TN; Hxb; Ten; TN-C; AI528729; cytotactin; tenascin-C; C130033P17Rik
NCBI Protein Information
tenascin
UniProt Protein Name
Tenascin
Protein Family
UniProt Gene Name
Tnc
UniProt Synonym Gene Names
TN; TN-C
Uniprot Description
TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4 C1|4 34.06 cM
Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; focal adhesion; membrane; proteinaceous extracellular matrix
Molecular Function: fibronectin binding; syndecan binding
Biological Process: axon regeneration in the peripheral nervous system; cell adhesion; negative regulation of cell adhesion; neuromuscular junction development; neuron projection development; osteoblast differentiation; positive regulation of cell proliferation; regulation of cell proliferation
Research Articles on TNC
Similar Products
Product Notes
The TNC tnc (Catalog #AAA2026510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tenascin C (TNC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tenascin C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TNC tnc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CVCEPGW KGPNCSEPDC PGNCNLRGQC LDGQCICDEG FTGEDCSQLA CPNDCNDQGR CVNGVCVCFE GYAGPDCGLE VCPVPCSEEH GMCVDGRCVC KDGFAGEDCN EPLCLNNCYN RGRCVENECV CDEGFTGEDC SELICPNDCF DRGRCINGTC YCEEGFTGED CGELTCPNDC QGRGQCEEGQ CVCNEGFAGA DCSEKRCPAD CHHRGRCLNG QCECDDGFTG ADCGDLQCPN GCSGHGRCVN GQCVCDEGYT GEDCSQRRCP NDCHNRGLCV QGKCICEQGF KGFDCSEMSC PNDCHQHGRC VNGMCICDDD YTGEDCRDRR CPRDCSQRGR CVDGQCICED GFTGPDCAEL SCPSDCHGHG RCVNGQCICH EGFTGKDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG. It is sometimes possible for the material contained within the vial of "Tenascin C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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