Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tenascin (TNC) Standard Curve.)
Mouse Tenascin (TNC) ELISA Kit | TNC elisa kit
Mouse Tenascin (TNC) ELISA Kit
Gene Names
Tnc; TN; Hxb; Ten; TN-C; AI528729; cytotactin; tenascin-C; C130033P17Rik
Reactivity
Mouse
Synonyms
Tenascin (TNC); Mouse Tenascin (TNC) ELISA Kit; TNC; HXB; MGC167029; TN; hexabrachion (tenascin C; cytotactin); hexabrachion (tenascin); tenascin C (hexabrachion); TNC elisa kit
Reactivity
Mouse
Specificity
Mouse Tenascin (TNC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNC. No significant cross-reactivity or interference between Mouse TNC and analogues was observed.
Sequence Length
2110
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tenascin (TNC) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tenascin (TNC) Standard Curve.)
Related Product Information for TNC elisa kit
Description: This Mouse Tenascin (TNC) ELISA Kit employs a two-site sandwich ELISA to quantitate TNC in samples. An antibody specific for TNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNC encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration.
Background: TNC encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tenascin
NCBI Official Synonym Full Names
tenascin C
NCBI Official Symbol
Tnc
NCBI Official Synonym Symbols
TN; Hxb; Ten; TN-C; AI528729; cytotactin; tenascin-C; C130033P17Rik
NCBI Protein Information
tenascin
UniProt Protein Name
Tenascin
Protein Family
UniProt Gene Name
Tnc
UniProt Synonym Gene Names
TN; TN-C
UniProt Entry Name
TENA_MOUSE
Uniprot Description
TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; focal adhesion; membrane; extracellular region; basement membrane
Molecular Function: syndecan binding; fibronectin binding
Biological Process: osteoblast differentiation; positive regulation of cell proliferation; negative regulation of cell adhesion; cell adhesion; axon regeneration in the peripheral nervous system; neurite development; neuromuscular junction development
Research Articles on TNC
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Product Notes
The Mouse TNC tnc (Catalog #AAA9716747) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716747 ELISA Kit recognizes Mouse TNC. It is sometimes possible for the material contained within the vial of "Tenascin (TNC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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