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Reactivity Chart
Goat pHuji Polyclonal Antibody | anti-pHuji antibody
anti-pHuji
Reactivity
Transfected cells, Red Fluorescent Protein
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Epitope affinity purified
Synonyms
pHuji; Polyclonal Antibody; anti-pHuji; red fluorescent protein antibody; anti-pHuji antibody
Host
Goat
Reactivity
Transfected cells, Red Fluorescent Protein
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Purity/Purification
Epitope affinity purified
Form/Format
?Polyclonal antibody supplied as a 100ul (3mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3mg/ml (varies by lot)
Applicable Applications for anti-pHuji antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM)
Application Notes
WB: 1:500-1:5,000
IF: 1:50-1:500
IHC-P: 1:50-1:500
IHC-F: 1:50-1:500
IEM: 1:50-1:500
IF: 1:50-1:500
IHC-P: 1:50-1:500
IHC-F: 1:50-1:500
IEM: 1:50-1:500
Conjugation
Unconjugated
Immunogen
Purified recombinant fluorescent protein produced in E Coli.
Preparation and Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Reactivity Chart
Reactivity Chart
Immunofluorescence (IF)
(Immunofluorescence -anti-pHuji Ab using COS-7 cells transduced with pHuji-Rab5a; cells were fixed with methanol andAnti-pHuji at 1/250;)
Immunofluorescence (IF)
(Immunofluorescence -anti-pHuji Ab using COS-7 cells transduced with pHuji-Rab5a; cells were fixed with methanol andAnti-pHuji at 1/250;)
Testing Data
(Anti-pHuji Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Testing Data
(Anti-pHuji Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Immunofluorescence (IF)
(Immunofluorescence -anti-pHuji Ab using COS-7 cells transduced with pHuji-Rab5a; cells were fixed with methanol and anti-pHuji at 1/250;)
Immunofluorescence (IF)
(Immunofluorescence -anti-pHuji Ab using COS-7 cells transduced with pHuji-Rab5a; cells were fixed with methanol and anti-pHuji at 1/250;)
Related Product Information for anti-pHuji antibody
Goat polyclonal antibody to pHuji (pHuji fluorescent protein). pHuji is a basic constitutively fluorescent protein engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. pHuji is a ~29kDa protein that is optimally excited at a 566nm and has a maximum of emission at 598nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Product Categories/Family for anti-pHuji antibody
Similar Products
Product Notes
The pHuji (Catalog #AAA448258) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The anti-pHuji reacts with Transfected cells, Red Fluorescent Protein and may cross-react with other species as described in the data sheet. AAA Biotech's pHuji can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM). WB: 1:500-1:5,000 IF: 1:50-1:500 IHC-P: 1:50-1:500 IHC-F: 1:50-1:500 IEM: 1:50-1:500. Researchers should empirically determine the suitability of the pHuji for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "pHuji, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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