Reactivity Chart
Goat mCherry Polyclonal Antibody
anti-mCherry
Reactivity
Transfected cells, Red Fluorescent Protein (dsRed), tdTomato and mCherry
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Epitope affinity purified
Synonyms
mCherry; Polyclonal Antibody; anti-mCherry; Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato antibody; anti-mCherry antibody
Host
Goat
Reactivity
Transfected cells, Red Fluorescent Protein (dsRed), tdTomato and mCherry
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27kDa by Western blot. This antibody recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Purity/Purification
Epitope affinity purified
Form/Format
Polyclonal antibody supplied as a 200ul (2mg/ml) aliquot in NaHCO3 100mM pH9; no preservatives. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.
Concentration
2mg/ml (varies by lot)
Applicable Applications for anti-mCherry antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM)
Application Notes
WB: 1:500-1:5,000
IF: 1:50-1:500
IHC-P: 1:50-1:500
IHC-F: 1:50-1:500
IEM: 1:50-1:500
IF: 1:50-1:500
IHC-P: 1:50-1:500
IHC-F: 1:50-1:500
IEM: 1:50-1:500
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide produced in E Coli.
Preparation and Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Reactivity Chart
Reactivity Chart
Testing Data
(Anti-mCherry Ab at 1/2,500 dilution; 293HEK cells transduced with mCherry Ad; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Testing Data
(Anti-mCherry Ab at 1/2,500 dilution; 293HEK cells transduced with mCherry Ad; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Related Product Information for anti-mCherry antibody
Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. mCherry is a ~27kDa protein that is optimally excited at a 587nm and has a maximum of emission at 610nm. It is a basic (constitutively fluorescent) red fluorescent protein and It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
Product Categories/Family for anti-mCherry antibody
Similar Products
Product Notes
The mCherry (Catalog #AAA448218) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The anti-mCherry reacts with Transfected cells, Red Fluorescent Protein (dsRed), tdTomato and mCherry and may cross-react with other species as described in the data sheet. AAA Biotech's mCherry can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM). WB: 1:500-1:5,000 IF: 1:50-1:500 IHC-P: 1:50-1:500 IHC-F: 1:50-1:500 IEM: 1:50-1:500. Researchers should empirically determine the suitability of the mCherry for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mCherry, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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