Western Blot (WB)
(TdT Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TdT antibody detected the TdT protein (arrow).)
Rabbit TdT Polyclonal Antibody | anti-DNTT antibody
TdT Antibody (C-term)
Western Blot (WB)
(TdT Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TdT antibody detected the TdT protein (arrow).)
Western Blot (WB)
(TdT Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TdT antibody detected the TdT protein (arrow).)
and encodes a template-independent DNA polymerase that catalyzes
the addition of deoxynucleotides to the 3'-hydroxyl terminus of
oligonucleotide primers. In vivo, the encoded protein is expressed
in a restricted population of normal and malignant pre-B and pre-T
lymphocytes during early differentiation, where it generates
antigen receptor diversity by synthesizing non-germ line elements
(N-regions) at the junctions of rearranged Ig heavy chain and T
cell receptor gene segments. Alternatively spliced transcript
variants encoding different isoforms of this gene have been
described.
O'Malley, D.P., et al. Haematologica 91(8):1139-1140(2006)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Thai, T.H., et al. J. Immunol. 173(6):4009-4019(2004)
Liu, L., et al. Am. J. Clin. Pathol. 121(6):810-815(2004)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; EC 2.7.7.31
Chromosomal Location of Human Ortholog: 10q23-q24
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; DNA nucleotidylexotransferase activity
Biological Process: DNA modification
Research Articles on DNTT
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Product Notes
The DNTT dntt (Catalog #AAA9212745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 438-467. The TdT Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TdT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the DNTT dntt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TdT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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