SNAPAP recombinant protein

SNAPAP, 1-136aa, Human, His tag, E Coli

Cat. Num. AAA203175

• Gene Names: SNAPIN; BLOS7; BORCS3; SNAPAP; BLOC1S7
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SNAPAP; 1-136aa; Human; His tag; E Coli; SNAP associated protein; SNAPIN; SNAP25BP; SNAP associated protein SNARE associated protein snapin; Synaptosomal associated protein 25 binding protein; SNAPAP recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 5 mM DTT, 2 mM EDTA, 0.2 M NaCl2, 40% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK
• Sequence Length: 136

• Applicable Applications for SNAPAP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SNAPAP recombinant protein

SNAPAP (SNAP associated protein) was enriched in neurons and exclusively located on synaptic vesicle membrane protein. SNAPAP is an important component of the neurotransmitter release process through its modulation of the sequential interactions between the SNAREs and synaptotagmin, which is a component of the SNARE complex that is required for synaptic vesicle docking and fusion. Recombinant human SNAPAP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SNAPAP recombinant protein

Neuroscience

References

Ilardi JM., et al. (1999). Nat Neurosci. 2 (2):119-124; Chheda MG., et al. (2001). Nat Cell Bioli. 3 (4):331-8;

NCBI and Uniprot Product Information

• NCBI GI #: 6912674
• NCBI GeneID: 23557
• NCBI Accession #: NP_036569
• NCBI GenBank Nucleotide #: NM_012437.5
• UniProt Accession #: O95295; Q5SXU8; D3DV56
• Molecular Weight: 17 kDa (156aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: SNARE-associated protein Snapin
• NCBI Official Synonym Full Names: SNAP associated protein
• NCBI Official Symbol: SNAPIN
• NCBI Official Synonym Symbols: BLOS7; BORCS3; SNAPAP; BLOC1S7
• NCBI Protein Information: SNARE-associated protein Snapin
• UniProt Protein Name: SNARE-associated protein Snapin
• UniProt Gene Name: SNAPIN
• UniProt Synonym Gene Names: BLOC1S7; SNAP25BP; SNAPAP; BLOC-1 subunit 7; SNAP-associated protein

NCBI Description

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:17182842, PubMed:18167355). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167).