Porcine Serotonin Transporter ELISA Kit | SERT elisa kit

Porcine Serotonin Transporter ELISA Kit

Cat. Num. AAA738979

• Gene Names: SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
• Reactivity: Porcine
• Synonyms: Serotonin Transporter; Porcine Serotonin Transporter ELISA Kit; SERT elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This SERT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SERT. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERT elisa kit

Principle of the Assay: SERT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SERT antibody and an SERT-HRP conjugate. The assay sample and buffer are incubated together with SERT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERT concentration since SERT from samples and SERT-HRP conjugate compete for the anti-SERT antibody binding site. Since the number of sites is limited, as more sites are occupied by SERT from the sample, fewer sites are left to bind SERT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERT concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SERT elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 58700442
• NCBI GeneID: 6532
• Molecular Weight: 74,979 Da
• NCBI Official Full Name: serotonin transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 4
• NCBI Official Symbol: SLC6A4
• NCBI Official Synonym Symbols: HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
• NCBI Protein Information: sodium-dependent serotonin transporter; 5HT transporter; serotonin transporter 1; Na+/Cl- dependent serotonin transporter; 5-hydroxytryptamine (serotonin) transporter; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• UniProt Protein Name: Sodium-dependent serotonin transporter
• Protein Family: Sodium-dependent serotonin transporter
• UniProt Gene Name: SLC6A4
• UniProt Synonym Gene Names: HTT; SERT; 5HTT
• UniProt Entry Name: SC6A4_HUMAN

NCBI Description

This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008]

Uniprot Description

SERT: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane; endomembrane system; endosome membrane; cytosol; lipid raft

Molecular Function: actin filament binding; serotonin transmembrane transporter activity; protein binding; protein homodimerization activity; myosin binding; serotonin:sodium symporter activity; cocaine binding; monoamine transmembrane transporter activity; syntaxin-1 binding; nitric-oxide synthase binding; Rab GTPase binding

Biological Process: circadian rhythm; response to drug; vasoconstriction; response to toxin; monoamine transport; positive regulation of cell cycle; thalamus development; social behavior; serotonin uptake; negative regulation of organ growth; negative regulation of synaptic transmission, dopaminergic; protein oligomerization; response to estradiol stimulus; memory; negative regulation of granule cell precursor proliferation; negative regulation of neuron differentiation; brain morphogenesis; response to hypoxia; sperm ejaculation; serotonin transport; transmembrane transport; protein homooligomerization; response to nutrient

Disease: Obsessive-compulsive Disorder; Anxiety