Rabbit anti-Human, Mouse TDRD7 Polyclonal Antibody | anti-TDRD7 antibody

TDRD7, CT (TDRD7, PCTAIRE2BP, Tudor domain-containing protein 7, PCTAIRE2-binding protein, Tudor repeat associator with PCTAIRE 2) (Azide free) (HRP)

Cat. Num. AAA6354649

• Gene Names: TDRD7; TRAP; CATC4; PCTAIRE2BP
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TDRD7; Polyclonal Antibody; CT (TDRD7; PCTAIRE2BP; Tudor domain-containing protein 7; PCTAIRE2-binding protein; Tudor repeat associator with PCTAIRE 2) (Azide free) (HRP); Tudor repeat associator with PCTAIRE 2; anti-TDRD7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TDRD7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TDRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-835 amino acids from the C-terminal region of human TDRD7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TDRD7 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TDRD7 antibody detected the TDRD7 protein (arrow).)

Western Blot (WB)

(TDRD7 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TDRD7 antibody detected the TDRD7 protein (arrow).)

Immunohistochemistry (IHC)

(TDRD7 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TDRD7 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TDRD7 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TDRD7 antibody

TDRD7, also known as Tudor domain containing protein 7, is a component of chromatoid bodies in spermatids (named processing bodies or P-bodies in somatic cells). It is found in a mRNP complex together with TDRD1, TDRD6 and DDX4. It is also found in a complex with CABLES1, PCTK2 and PIWIL1. TDRD7 has been shown to interact with histone H3 tri-methylated at K9 in vitro.

Product Categories/Family for anti-TDRD7 antibody

Antibodies; Binding Proteins

References

Lim, J., et al. Cell 125(4):801-814(2006). Conte, N., et al. Oncogene 22(50):8102-8116(2003). Lauffart, B., et al. Biochem. J. 363 (PT 1), 195-200 (2002): Yamochi, T., et al. Biochem. Biophys. Res. Commun. 286(5):1045-1050(2001). Hirose, T., et al. Eur. J. Biochem. 267(7):2113-2121(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 112293287
• NCBI GeneID: 23424
• NCBI Accession #: NP_055105.2
• NCBI GenBank Nucleotide #: NM_014290.2
• UniProt Accession #: Q8NHU6; Q5VV27; Q96JT1; Q9UFF0; Q9Y2M3; A6NCI6; B2RBX3; B4DG99; B4DXF7; E7EQD4
• Molecular Weight: 47,539 Da
• NCBI Official Full Name: tudor domain-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: tudor domain containing 7
• NCBI Official Symbol: TDRD7
• NCBI Official Synonym Symbols: TRAP; CATC4; PCTAIRE2BP
• NCBI Protein Information: tudor domain-containing protein 7
• UniProt Protein Name: Tudor domain-containing protein 7
• Protein Family: Tudor domain-containing protein
• UniProt Gene Name: TDRD7
• UniProt Synonym Gene Names: PCTAIRE2BP; Trap
• UniProt Entry Name: TDRD7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Tudor family of proteins. This protein contains conserved Tudor domains and LOTUS domains. It is a component of RNA granules, which function in RNA processing. Mutations in this gene have been associated with cataract formation in mouse and human. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]