Rabbit anti-Human, Mouse TDRD7 Polyclonal Antibody | anti-TDRD7 antibody

TDRD7, CT (TDRD7, PCTAIRE2BP, Tudor domain-containing protein 7, PCTAIRE2-binding protein, Tudor repeat associator with PCTAIRE 2)

Cat. Num. AAA6010857

• Gene Names: TDRD7; TRAP; CATC4; CTRCT36; PCTAIRE2BP; RP11-508D10.1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TDRD7; Polyclonal Antibody; CT (TDRD7; PCTAIRE2BP; Tudor domain-containing protein 7; PCTAIRE2-binding protein; Tudor repeat associator with PCTAIRE 2); Anti -TDRD7; anti-TDRD7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TDRD7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: TDRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-835 amino acids from the C-terminal region of human TDRD7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TDRD7 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TDRD7 antibody detected the TDRD7 protein (arrow).)

Western Blot (WB)

(TDRD7 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TDRD7 antibody detected the TDRD7 protein (arrow).)

Immunohistochemistry (IHC)

(TDRD7 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TDRD7 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TDRD7 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TDRD7 antibody

TDRD7, also known as Tudor domain containing protein 7, is a component of chromatoid bodies in spermatids (named processing bodies or P-bodies in somatic cells). It is found in a mRNP complex together with TDRD1, TDRD6 and DDX4. It is also found in a complex with CABLES1, PCTK2 and PIWIL1. TDRD7 has been shown to interact with histone H3 tri-methylated at K9 in vitro.

Product Categories/Family for anti-TDRD7 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 112293287
• NCBI GeneID: 23424
• NCBI Accession #: NP_055105.2
• NCBI GenBank Nucleotide #: NM_014290.2
• UniProt Accession #: Q8NHU6; Q5VV27; Q96JT1; Q9UFF0; Q9Y2M3; A6NCI6; B2RBX3; B4DG99; B4DXF7; E7EQD4
• Molecular Weight: 123,586 Da
• NCBI Official Full Name: tudor domain-containing protein 7
• NCBI Official Synonym Full Names: tudor domain containing 7
• NCBI Official Symbol: TDRD7
• NCBI Official Synonym Symbols: TRAP; CATC4; CTRCT36; PCTAIRE2BP; RP11-508D10.1
• NCBI Protein Information: tudor domain-containing protein 7; PCTAIRE2-binding protein; tudor repeat associator with PCTAIRE 2; tudor repeat associator with PCTAIRE-2
• UniProt Protein Name: Tudor domain-containing protein 7
• Protein Family: Tudor domain-containing protein
• UniProt Gene Name: TDRD7
• UniProt Synonym Gene Names: PCTAIRE2BP; Trap
• UniProt Entry Name: TDRD7_HUMAN

NCBI Description

This gene encodes a component of cytoplasmic RNA granules which are involved in determining the fate of mRNAs. Mutation in this gene has been associated with pediatric cataracts. Mutation of the similar gene in mice has been associated with cataracts, glaucoma and a block in spermatogenesis. [provided by RefSeq, May 2011]

Uniprot Description

TDRD7: Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. Required for lens transparency during lens development, by regulating translation of genes such as CRYBB3 and HSPB1 in the developing lens. Also required during spermatogenesis. Defects in TDRD7 are the cause of cataract congenital autosomal recessive type 4 (CATC4). An opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Belongs to the TDRD7 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function: mRNA binding; protein binding; protein N-terminus binding

Biological Process: lens morphogenesis in camera-type eye; spermatogenesis; germ cell development

Disease: Cataract 36