CD147 / EMMPRIN / Basigin Recombinant Protein | CD147 / EMMPRIN recombinant protein

Recombinant Human CD147 / EMMPRIN / Basigin Protein (Fc tag)

Cat. Num. AAA2546437

• Gene Names: BSG; OK; 5F7; TCSF; CD147; EMMPRIN
• Purity: > 97 % as determined by SDS-PAGE and SEC-HPLC Analysis.
• Synonyms: CD147 / EMMPRIN / Basigin; Recombinant Human CD147 / EMMPRIN / Basigin Protein (Fc tag); BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN; CD147 / EMMPRIN recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE and SEC-HPLC Analysis.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 269

• Application Notes: The recombinant mature human CD147/Fc chimera protein consists of 422 amino acids and has a calculated molecular mass of 46.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 58-65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD147 / EMMPRIN recombinant protein

Background: CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

Description: A DNA sequence encoding the extracellular domain (Met 1-His 205) of human CD147 (NP_940991.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 38372925
• NCBI GeneID: 682
• NCBI Accession #: NP_940991.1
• NCBI GenBank Nucleotide #: NM_198589.2
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: basigin isoform 2
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin
• UniProt Protein Name: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell-cell adherens junction; focal adhesion; Golgi membrane; integral to plasma membrane; membrane; mitochondrion; plasma membrane

Molecular Function: monocarboxylic acid transmembrane transporter activity; protein binding

Biological Process: cell surface receptor linked signal transduction; extracellular matrix disassembly; extracellular matrix organization and biogenesis; leukocyte migration; pyruvate metabolic process

Disease: Blood Group--ok