Rabbit TDAG8 Polyclonal Antibody | anti-Gpr65 antibody

TDAG8 (Psychosine Receptor, G-protein Coupled Receptor 65, T-cell Death-associated Gene 8 Protein, GPR65)

Cat. Num. AAA646775

• Gene Names: Gpr65; Dig1; TDAG8; Gpcr25
• Reactivity: Human, Monkey, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TDAG8; Polyclonal Antibody; TDAG8 (Psychosine Receptor; G-protein Coupled Receptor 65; T-cell Death-associated Gene 8 Protein; GPR65); Anti -TDAG8 (Psychosine Receptor; anti-Gpr65 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPR65. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-Gpr65 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-2ug/ml; Immunohistochemistry (paraffin): 20ug/ml
• Immunogen: Synthetic peptide corresponding to aa200-250 of human GPR65.
• Positive Control: Human kidney lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of human GPR65 using human kidney lysate in the 1) absence and 2) presence of immunizing peptide using MBS646775 at 1ug/ml.)

Immunohistochemistry (IHC)

(IHC analysis of human tonsil using MBS646775 at 20ug/ml.)

Related Product Information for anti-Gpr65 antibody

GPR65 also known as TDAG8 is a transmembrane protein belonging to the G protein coupled receptor super family with 337aa and 90% similarity and 81% identity in the 7 transmembrane domains of its mouse homolog. It has 3 potential N-linked glycosylation sites and is expressed predominantly in lymphoid organs and cancer tissues with increased expression during activation-induced death of T-cell hybridomas. Two major transcripts of 4.5kb and 1.8kb are found in peripheral blood leukocytes and functions as a novel proton-sensing receptor. It couples to adenylyl cyclase and psychosine and is also actively involved in the establishment of immunological autotolerance. GPR65 has a role in cellular transformation by RhoA activation and actin rearrangement under conditions accompanying tissue acidosis.

Product Categories/Family for anti-Gpr65 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 1103873
• NCBI GeneID: 14744
• UniProt Accession #: Q61038; Q921N3
• Molecular Weight: 39,388 Da
• NCBI Official Full Name: TDAG8
• NCBI Official Synonym Full Names: G-protein coupled receptor 65
• NCBI Official Symbol: Gpr65
• NCBI Official Synonym Symbols: Dig1; TDAG8; Gpcr25
• NCBI Protein Information: psychosine receptor; G-protein coupled receptor 25; T-cell death-associated gene 8 protein
• UniProt Protein Name: Psychosine receptor
• Protein Family: Psychosine receptor
• UniProt Gene Name: Gpr65
• UniProt Synonym Gene Names: Gpcr25; Tdag8
• UniProt Entry Name: PSYR_MOUSE

Uniprot Description

Function: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation-induced cell death or differentiation of T-cells.

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Detected in thymus and at low levels in spleen.

Induction: By glucocorticoids, during apoptosis of immature thymocytes.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.