Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 13 (MED13) Standard Curve.)
Mouse Mediator Of RNA Polymerase II Transcription Subunit 13 (MED13) ELISA Kit | MED13 elisa kit
Mouse Mediator Of RNA Polymerase II Transcription Subunit 13 (MED13) ELISA Kit
Gene Names
Med13; Thrap1; Trap240; D030023K18; 1110067M05Rik
Reactivity
Mouse
Synonyms
Mediator Of RNA Polymerase II Transcription Subunit 13 (MED13); Mouse Mediator Of RNA Polymerase II Transcription Subunit 13 (MED13) ELISA Kit; MED13; ARC250; DRIP250; HSPC221; KIAA0593; THRAP1; TRAP240; activator-recruited cofactor 250 kDa component; mediator of RNA polymerase transcription; subunit 13 homolog; thyroid hormone receptor associate; MED13 elisa kit
Reactivity
Mouse
Specificity
Mouse Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MED13. No significant cross-reactivity or interference between Mouse MED13 and analogues was observed.
Sequence Length
2171
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 13 (MED13) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mediator of RNA polymerase II transcription subunit 13 (MED13) Standard Curve.)
Related Product Information for MED13 elisa kit
Description: This Mouse Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13 in samples. An antibody specific for MED13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MED13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
Background: MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 13
NCBI Official Synonym Full Names
mediator complex subunit 13
NCBI Official Symbol
Med13
NCBI Official Synonym Symbols
Thrap1; Trap240; D030023K18; 1110067M05Rik
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 13
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 13
Protein Family
UniProt Gene Name
Med13
UniProt Synonym Gene Names
Kiaa0593; Thrap1; Trap240; Trap240
UniProt Entry Name
MED13_MOUSE
Research Articles on MED13
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Product Notes
The Mouse MED13 med13 (Catalog #AAA9717681) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717681 ELISA Kit recognizes Mouse MED13. It is sometimes possible for the material contained within the vial of "Mediator Of RNA Polymerase II Transcription Subunit 13 (MED13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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