Rabbit TCP1 Polyclonal Antibody | anti-TCP1 antibody

TCP1 Antibody

Cat. Num. AAA9402136

• Gene Names: TCP1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TCP1; Polyclonal Antibody; TCP1 Antibody; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha; anti-TCP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TCP1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 556

• Applicable Applications for anti-TCP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TCP1.
• Target Name: TCP1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TCP1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testis using TCP1 antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using TCP1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-TCP1 antibody

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Product Categories/Family for anti-TCP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 57863257
• NCBI GeneID: 6950
• NCBI Accession #: NP_110379.2
• NCBI GenBank Nucleotide #: NM_030752.2
• UniProt Accession #: P17987; Q15556; Q5TCM3; E1P5B2
• Molecular Weight: 60,344 Da
• NCBI Official Full Name: T-complex protein 1 subunit alpha isoform a
• NCBI Official Synonym Full Names: t-complex 1
• NCBI Official Symbol: TCP1
• NCBI Official Synonym Symbols: CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
• NCBI Protein Information: T-complex protein 1 subunit alpha
• UniProt Protein Name: T-complex protein 1 subunit alpha
• Protein Family: T-complex protein
• UniProt Gene Name: TCP1
• UniProt Synonym Gene Names: CCT1; CCTA; TCP-1-alpha
• UniProt Entry Name: TCPA_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]

Uniprot Description

CCT-alpha: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Belongs to the TCP-1 chaperonin family.

Protein type: Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 6q25.3-q26

Cellular Component: Golgi apparatus; chaperonin-containing T-complex; microtubule; centrosome; pericentriolar material; acrosome; nuclear heterochromatin; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida