Mouse anti-Human TCP1 Monoclonal Antibody | anti-TCP1 antibody

Anti-TCP1 Antibody (clone 2B2-D6)

Cat. Num. AAA2400858

• Gene Names: TCP1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: TCP1; Monoclonal Antibody; Anti-TCP1 Antibody (clone 2B2-D6); Rat Monoclonal [clone 91a] (IgG2a) to Mouse TCP1; CCTA; CCT-alpha; D6S230E; T-complex 1; TCP-1-alpha; Tailless complex polypeptide 1; CCT1; anti-TCP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 2B2-D6
• Specificity: Human T-complex 1
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Sequence Length: 556

• Applicable Applications for anti-TCP1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 10 ug/ml; IHC-P: 10 ug/ml
• Immunogen: TCP1 (AAH00665, 1 a.a. ~ 557 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TCP1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to TCP1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(TCP1 monoclonal antibody (M01), clone 2B2-D6 Western blot of TCP1 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged TCP1 is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-TCP1 antibody

TCP1 is a molecular chaperone that is a member of the chaperonincontaining TCP1 complex (CCT), also known as the TCP1 ring complex(TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

NCBI and Uniprot Product Information

• NCBI GI #: 57863257
• NCBI GeneID: 6950
• NCBI Accession #: NP_110379.2
• NCBI GenBank Nucleotide #: NM_030752.2
• UniProt Accession #: P17987; Q15556; Q5TCM3; E1P5B2
• Molecular Weight: 60,344 Da
• NCBI Official Full Name: T-complex protein 1 subunit alpha isoform a
• NCBI Official Synonym Full Names: t-complex 1
• NCBI Official Symbol: TCP1
• NCBI Official Synonym Symbols: CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
• NCBI Protein Information: T-complex protein 1 subunit alpha
• UniProt Protein Name: T-complex protein 1 subunit alpha
• Protein Family: T-complex protein
• UniProt Gene Name: TCP1
• UniProt Synonym Gene Names: CCT1; CCTA; TCP-1-alpha

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]

Uniprot Description

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.